Ficolin-2血清水平和FCN2基因变异与印度内脏利什曼病的关联
Association of Ficolin-2 Serum Levels and FCN2 Genetic Variants with Indian Visceral Leishmaniasis.
作者信息
Mishra Anshuman, Antony Justin S, Sundaravadivel Pandarisamy, Tong Hoang Van, Meyer Christian G, Jalli Reshma D, Velavan Thirumalaisamy P, Thangaraj Kumarasamy
机构信息
Council of Scientific and Industrial Research-Centre for Cellular and Molecular Biology, Hyderabad, India.
Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
出版信息
PLoS One. 2015 May 12;10(5):e0125940. doi: 10.1371/journal.pone.0125940. eCollection 2015.
BACKGROUND
Visceral leishmaniasis (VL), one of the neglected tropical diseases, is endemic in the Indian subcontinent. Ficolins are circulating serum proteins of the lectin complement system and involved in innate immunity.
METHODS
We have estimated ficolin-2 serum levels and analyzed the functional variants of the encoding gene FCN2 in 218 cases of VL and in 225 controls from an endemic region of India.
RESULTS
Elevated levels of serum ficolin-2 were observed in VL cases compared to the controls (adjusted P<0.0001). The genetic analysis revealed that the FCN2 structural variant +6359 C>T (p.T236M) was associated with VL (OR=2.2, 95% CI=1.23-7.25, P=0.008) and with high ficolin-2 serum levels. We also found that the FCN2*AAAC haplotype occurred more frequently among healthy controls when compared to cases (OR=0.59, 95%CI=0.37-0.94, P=0.023).
CONCLUSIONS
Our findings indicate that the FCN2 variant +6359C>T is associated with the occurrence of VL and that ficolin-2 serum levels are elevated in Leishmania infections.
背景
内脏利什曼病（VL）是一种被忽视的热带疾病，在印度次大陆流行。纤维胶凝蛋白是凝集素补体系统的循环血清蛋白，参与固有免疫。
方法
我们估计了218例VL患者和来自印度一个流行地区的225名对照者的纤维胶凝蛋白-2血清水平，并分析了编码基因FCN2的功能变异。
结果
与对照组相比，VL患者血清纤维胶凝蛋白-2水平升高（校正P<0.0001）。基因分析显示，FCN2结构变异+6359 C>T（p.T236M）与VL相关（OR=2.2，95%CI=1.23-7.25，P=0.008），且与高纤维胶凝蛋白-2血清水平相关。我们还发现，与病例相比，FCN2*AAAC单倍型在健康对照者中出现的频率更高（OR=0.59，95%CI=0.37-0.94，P=0.023）。
结论
我们的研究结果表明，FCN2变异+6359C>T与VL的发生相关，且在利什曼原虫感染中纤维胶凝蛋白-2血清水平升高。
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