Lv X Y, Sun W, Su R, Li D, Wang Q Z, Musa H H, Chen L, Zhang Y F, Wu W Z
Animal Science and Technology College, Yangzhou University, Yangzhou, China.
Animal Science and Technology College, Yangzhou University, Yangzhou, China
Genet Mol Res. 2015 Apr 13;14(2):3244-56. doi: 10.4238/2015.April.13.3.
The aim of the current study was to investigate the effects of Yes-associated protein 1 (YAP1) gene expression after birth on the development of muscle and the relationship between YAP1 and myostatin (MSTN) and myogenin (MyoG). Reverse transcription polymerase chain reaction was used to analyze the trends in YAP1, MSTN, and MyoG temporal and spatial expression levels in various skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and across 3 different growth stages (i.e., 2 days old, 2 and 6 months old) of Hu Sheep. The results showed that YAP1 expression was significantly different in the skeletal muscles of sheep; the expression level gradually increased with age; it was highly expressed in the gastrocnemius muscle and minimally expressed in the longissimus dorsi muscle. MSTN, a negative regulator of skeletal muscle development, was minimally expressed in the soleus muscle and might be related to the enlargement of muscle fiber diameter. MyoG, an important factor in regulating skeletal muscle development, was minimally expressed in the longissimus dorsi muscle and extensor digitorum longus, and highly expressed in the gastrocnemius and soleus muscles; it might inhibit the enlargement of muscle fiber diameter after birth. YAP1 expression was significantly (P < 0.05) or extremely significantly (P < 0.01) and positively correlated with MSTN and MyoG at 2 days old, 2 and 6 months old. YAP1 expression was related to muscle fiber development after birth and might be a candidate gene for the regulation of muscle growth.
本研究旨在探讨出生后Yes相关蛋白1（YAP1）基因表达对肌肉发育的影响，以及YAP1与肌肉生长抑制素（MSTN）和生肌调节因子（MyoG）之间的关系。采用逆转录聚合酶链反应分析YAP1、MSTN和MyoG在湖羊不同骨骼肌（即背最长肌、比目鱼肌、腓肠肌和趾长伸肌）以及3个不同生长阶段（即2日龄、2月龄和6月龄）的时空表达水平变化趋势。结果表明，YAP1在绵羊骨骼肌中的表达存在显著差异；表达水平随年龄增长逐渐升高；在腓肠肌中高表达，在背最长肌中低表达。MSTN作为骨骼肌发育的负调控因子，在比目鱼肌中低表达，可能与肌纤维直径增大有关。MyoG作为调节骨骼肌发育的重要因子，在背最长肌和趾长伸肌中低表达，在腓肠肌和比目鱼肌中高表达；可能在出生后抑制肌纤维直径增大。在2日龄、2月龄和6月龄时，YAP1表达与MSTN和MyoG呈显著（P<0.05）或极显著（P<0.01）正相关。YAP1表达与出生后肌纤维发育有关，可能是调控肌肉生长的候选基因。
