Sun W, Su R, Li D, Musa H H, Kong Y, Ding J T, Ma Y H, Chen L, Zhang Y F, Wu W Z
Animal Science and Technology College, Yangzhou University, Yangzhou, China
Animal Science and Technology College, Yangzhou University, Yangzhou, China.
Genet Mol Res. 2014 Apr 14;13(2):2772-83. doi: 10.4238/2014.April.14.6.
In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.01 < P < 0.05), one-month old (0.01 < P < 0.05), and three month-old (P < 0.01) stages. IGF-I gene expression in male longissimus muscles was higher than that of females at all growth stages, except for the three month-old stage. There was no significant difference (P > 0.05) between males and females at any growth stage in expression of the MyoG gene. MyoG gene expression in male longissimus muscles tended to be higher than that of females at all growth stages, except for the six month-old stage. IGF-I gene expression was significantly and positively correlated with live weight (P < 0.01) and carcass weight (0.01< P < 0.05), and was non-significantly positively correlated with net meat weight (P > 0.05). In contrast, MyoG gene expression was non-significantly and positively correlated with live weight, carcass, and net meat weight (P > 0.05). Carcass traits showed highly significant positive correlations (P < 0.01). Furthermore, expressions of IGF-I and MyoG genes showed highly significant positive correlations (P < 0.01). We conclude that the expressions of IGF-I and MyoG genes are significantly and positively correlated with early muscle traits of Hu sheep.
在本研究中，应用实时聚合酶链反应分析不同生长阶段湖羊背最长肌中IGF-I和MyoG基因的表达及其与肉品质性状的关联。湖羊IGF-I基因在2日龄（0.01 < P < 0.05）、1月龄（0.01 < P < 0.05）和3月龄（P < 0.01）时，雄性和雌性之间的表达差异显著。除3月龄阶段外，在所有生长阶段雄性背最长肌中IGF-I基因的表达均高于雌性。在任何生长阶段，雄性和雌性MyoG基因的表达均无显著差异（P > 0.05）。除6月龄阶段外，在所有生长阶段雄性背最长肌中MyoG基因的表达均倾向于高于雌性。IGF-I基因表达与活重（P < 0.01）和胴体重（0.01 < P < 0.05）显著正相关，与净肉重呈非显著正相关（P > 0.05）。相比之下，MyoG基因表达与活重、胴体重和净肉重呈非显著正相关（P > 0.05）。胴体性状呈极显著正相关（P < 0.01）。此外，IGF-I和MyoG基因的表达呈极显著正相关（P < 0.01）。我们得出结论，IGF-I和MyoG基因的表达与湖羊早期肌肉性状显著正相关。
