Lu L, Li S M, Zhang L, Liu X Q, Li D Y, Zhao X L, Liu Y P
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an, China.
Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science, Nanchang, China.
Genet Mol Res. 2015 Apr 13;14(2):3330-7. doi: 10.4238/2015.April.13.12.
The objective of this study was to evaluate the effect of vitamin D3 (VD3) on the regulation of chicken intestinal β-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group. At 4, 24, and 48 h post-injection, intestines were collected and RNA was isolated to measure the chicken β-defensin genes with putative vitamin D responsive elements using quantitative polymerase chain reaction. Expressions of all 7 chicken β-defensin genes was detectable in the intestines. Significant increases in GAL-6, -7 and -9 were found following LPS injection treatment at 4, 24, and 48 h post-injection, respectively, whereas VD3 injection did not affect the expression of any investigated genes under normal conditions. However, the expression of GAL-4, -5, -6, and -10 were synergistically upregulated by VD3 in combination with LPS. These results suggest that VD3 enhances the immune immunity during LPS challenge by inducing the expression of chicken β-defensin genes when birds are exposed to immune stressors.
本研究的目的是评估维生素D3（VD3）在正常和脂多糖（LPS）条件下对鸡肠道β-防御素基因调控的影响。实验采用了四个处理组，包括阴性对照组、VD3注射组、LPS注射组以及VD3注射和LPS注射联合组。在注射后4、24和48小时，采集肠道并分离RNA，使用定量聚合酶链反应测量具有假定维生素D反应元件的鸡β-防御素基因。在肠道中可检测到所有7种鸡β-防御素基因的表达。分别在注射后4、24和48小时，LPS注射处理后GAL-6、-7和-9显著增加，而在正常条件下，VD3注射不影响任何研究基因的表达。然而，VD3与LPS联合可协同上调GAL-4、-5、-6和-10的表达。这些结果表明，当鸡暴露于免疫应激源时，VD3通过诱导鸡β-防御素基因的表达增强LPS攻击期间的免疫免疫力。
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