Sukjamnong S, Santiyanont R
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand
Genet Mol Res. 2015 Apr 27;14(2):3984-94. doi: 10.4238/2015.April.27.13.
Fimbristylis ovata has been long used as a traditional medicine for chronic inflammatory diseases; however, there are no data regarding its anti-inflammatory properties. In this study, we investigated the effects of F. ovata extracts on the secretion of pro-inflammatory cytokines, cell adhesion molecule, and receptor for advanced glycation end-products (RAGE) in lipopolysaccharide-stimulated cells. F. ovata was extracted using the maceration method with 3 different solvents: ethanol, methanol, and water. The effect of F. ovata extracts on cell viability was evaluated using the MTT assay. Pro-inflammatory cytokines and cell adhesion molecules were investigated by reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay. Upon incubation with F. ovata extracts up to 100 mg/mL, cell viability was more than 80%. F. ovata extracts could inhibit interleukin-6 level and gene expression as well as the RAGE gene in the monocytic cell lineU937. Moreover, the results showed that vascular cell adhesion molecule 1 secretion and gene expression were decreased when lipopolysaccharide-activated brain endothelial cells (bEnd.3) were treated with F. ovata extracts. Therefore, the anti-inflammatory activity of F. ovata extracts may result from their inhibitory actions via the RAGE signaling pathway.
卵叶飘拂草长期以来被用作治疗慢性炎症性疾病的传统药物；然而，关于其抗炎特性尚无相关数据。在本研究中，我们调查了卵叶飘拂草提取物对脂多糖刺激的细胞中促炎细胞因子、细胞粘附分子和晚期糖基化终产物受体（RAGE）分泌的影响。卵叶飘拂草采用浸渍法用3种不同溶剂提取：乙醇、甲醇和水。使用MTT法评估卵叶飘拂草提取物对细胞活力的影响。通过逆转录-聚合酶链反应和酶联免疫吸附测定法研究促炎细胞因子和细胞粘附分子。用高达100mg/mL的卵叶飘拂草提取物孵育后，细胞活力超过80%。卵叶飘拂草提取物可抑制单核细胞系U937中的白细胞介素-6水平和基因表达以及RAGE基因。此外，结果表明，用卵叶飘拂草提取物处理脂多糖激活的脑内皮细胞（bEnd.3）时，血管细胞粘附分子1的分泌和基因表达降低。因此，卵叶飘拂草提取物的抗炎活性可能源于其通过RAGE信号通路的抑制作用。
