Zhao C H, Cheng G-C, He R L, Hong Y, Wan Q L, Wang Z-Z, Pan Z-Y
Department of Cardiology, Henan University Huaihe Hospital, Kaifeng, Henan, China.
Department of Cardiology, Henan University Huaihe Hospital, Kaifeng, Henan, China
Genet Mol Res. 2015 Apr 27;14(2):4027-34. doi: 10.4238/2015.April.27.17.
The aim of this study was to investigate the expression level of microRNA-499 and its clinical significance in serum of patients with acute myocardial infarction (AMI). We recruited 59 patients with AMI and 60 healthy individuals undergoing physical examination in our hospital during the same period as controls. Peripheral blood was drawn in the morning on the same day of microRNA extraction. The expression level of microRNA-499 was analyzed by real-time fluorescent quantitative polymerase chain reaction (qPCR). The sensitivity and specificity of the clinical diagnosis of AMI were analyzed by a receiver operating characteristic (ROC) curve. Fluorescent qPCR analysis showed that the expression of microRNA-499 in serum of patients with AMI was significantly higher than in controls (P < 0.05). MicroRNA-499 was detected in blood serum 3 h post-AMI, reaching a peak after 12 h and declining after 15 h. The area under the ROC curve (AUC) for the gold standard cardiac troponin I (cTnI) was 0.971 [95% confidence interval (CI): 0.951-1.000], and for the microRNA-499, AUC = 0.915 (95%CI: 0.826-1.000). When the microRNA-499 levels in patient and control (> 1.5) sera were compared, the sensitivity of microRNA-499 in judging AMI was found to be 86.37% and the specificity was 93.47%. Our results demonstrated that the expression levels of microRNA-499 in serum of patients with AMI were abnormal. Its high sensitivity and specificity for the diagnosis of AMI suggest that it would be useful as an auxiliary index for clinical diagnosis of AMI.
本研究旨在探讨急性心肌梗死（AMI）患者血清中微小RNA-499的表达水平及其临床意义。我们招募了59例AMI患者和60例同期在我院接受体检的健康个体作为对照。在提取微小RNA的同一天上午采集外周血。采用实时荧光定量聚合酶链反应（qPCR）分析微小RNA-499的表达水平。通过受试者工作特征（ROC）曲线分析AMI临床诊断的敏感性和特异性。荧光qPCR分析显示，AMI患者血清中微小RNA-499的表达明显高于对照组（P<0.05）。AMI后3小时在血清中检测到微小RNA-499，12小时后达到峰值，15小时后下降。金标准心肌肌钙蛋白I（cTnI）的ROC曲线下面积（AUC）为0.971[95%置信区间（CI）：0.951-1.000]，微小RNA-499的AUC=0.915（95%CI：0.826-1.000）。比较患者和对照（>1.5）血清中微小RNA-499水平时，发现微小RNA-499判断AMI的敏感性为86.37%，特异性为93.47%。我们的结果表明，AMI患者血清中微小RNA-499的表达水平异常。其对AMI诊断的高敏感性和特异性表明，它可作为AMI临床诊断的辅助指标。
