He P, Chen X L, Ding Y P
Emergency Center of the People's Hospital of Hainan Province, Haikou, Hainan, China.
Emergency Center of the People's Hospital of Hainan Province, Haikou, Hainan, China
Genet Mol Res. 2015 Apr 27;14(2):4142-9. doi: 10.4238/2015.April.27.29.
We investigated the association between polymorphisms in the adiponectin gene (APM-1) and atherosclerotic cerebral infarction (ACI) in a Chinese Han population of Hainan Province. Polymerase chain reaction-restriction fragment length polymorphism and gene sequencing were used to analyze the distribution of APM-1 +45T/G and +276G/T genotypes and their alleles in 120 ACI patients and 120 healthy controls. No statistical correlation was found in the frequency and distribution of the genotype 45T/G between the ACI group and the control group. Genotypic frequencies of GG, GT, and TT at the APM-1 +276 locus were 70.0% (84/120), 25.0% (30/120), and 5.0% (6/120), respectively, in the ACI group, while these values were 52.5% (63/120), 37.5% (45/120), and 10.0% (12/120), respectively, in the control group. The frequency of the G allele was 82.5% (198/240) in the ACI group and 71.25% (171/240) in the control group. The T allele frequency was 17.5% (42/240) in the ACI group and 28.75% (69/240) in the control group. Polymorphisms at the APM-1 -276 locus in the case-controlled groups showed significant differences in the genotype distribution and al-lele frequency between the 2 groups (P = 0.041). The occurrence of ACI in the Hainan Chinese Han population may be associated with +276G/T polymorphisms but not with +45T/G polymorphisms in the APM-1 gene.
我们在海南省的中国汉族人群中研究了脂联素基因（APM-1）多态性与动脉粥样硬化性脑梗死（ACI）之间的关联。采用聚合酶链反应-限制性片段长度多态性和基因测序方法，分析120例ACI患者和120例健康对照者中APM-1 +45T/G和+276G/T基因型及其等位基因的分布。ACI组与对照组之间45T/G基因型的频率和分布无统计学相关性。APM-1 +276位点的GG、GT和TT基因型频率在ACI组分别为70.0%（84/120）、25.0%（30/120）和5.0%（6/120），而在对照组分别为52.5%（63/120）、37.5%（45/120）和10.0%（12/120）。G等位基因频率在ACI组为82.5%（198/240），在对照组为71.25%（171/240）。T等位基因频率在ACI组为17.5%（42/240），在对照组为28.75%（69/240）。病例对照研究组中APM-1 -276位点的多态性在两组间基因型分布和等位基因频率存在显著差异（P = 0.041）。海南中国汉族人群中ACI的发生可能与APM-1基因的+276G/T多态性有关，而与+45T/G多态性无关。
