Wang X-L, Li Y, Liao L, Bai C-J, Wang Z-Y
Key Laboratory of Protection and Developmental Utilization of Tropical Crop Germplasm Resources of Hainan University, Ministry of Education/College of Agronomy, Hainan University, Haikou, China.
College of Forestry, Henan Agricultural University, Zhengzhou, China.
Genet Mol Res. 2015 May 11;14(2):4696-702. doi: 10.4238/2015.May.11.2.
Axonopus compressus (Sw.) Beauv. is a perennial herb widely used as a garden lawn grass. In this study, we used Roche 454 pyrosequencing, combined with the magnetic bead enrichment method FIASCO, to isolate simple sequence repeat markers from the A. compressus genome. A total of 1942 microsatellite loci were identified, with 53,193 raw sequencing reads. One hundred micro-satellite loci were selected to test the primer amplification efficiency in 24 individuals; 14 primer pairs yielded polymorphic amplification products. The number of observed alleles ranged from two to six, with an average of 3.5. Shannon's Information index values ranged from 0.169 to 0.650, with an average of 0.393. Nei's genetic diversity values ranged from 0.108 to 0.457, with an average of 0.271. This first set of microsatellite markers developed for Axonopus will assist in the development of molecular marker-assisted breeding and the assessment of genetic diversity in A. compressus.
地毯草（Axonopus compressus (Sw.) Beauv.）是一种多年生草本植物，广泛用作园林草坪草。在本研究中，我们使用罗氏454焦磷酸测序技术，并结合磁珠富集法FIASCO，从地毯草基因组中分离简单序列重复标记。共鉴定出1942个微卫星位点，原始测序读数为53193条。选择100个微卫星位点在24个个体中测试引物扩增效率；14对引物产生了多态性扩增产物。观察到的等位基因数从2到6不等，平均为3.5。香农信息指数值从0.169到0.650不等，平均为0.393。内氏遗传多样性值从0.108到0.457不等，平均为0.271。这套首次为地毯草开发的微卫星标记将有助于分子标记辅助育种的发展以及地毯草遗传多样性的评估。
