Yang Xuemei, Du Jianxiu, Li Yinhuan
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Talanta. 2015 Aug 15;141:207-11. doi: 10.1016/j.talanta.2015.04.003. Epub 2015 Apr 9.
A cost-efficient and portable device for detecting sulfide at submicromolar level was fabricated by in situ integrating gas-permeable porous tube isolation and long path absorbance detection. The device consisted of a pair of petri dish, having a diametrically strung porous membrane tube in the top cover. The ends of the tube were terminated by a light emitting diode and a photodiode via plugging acrylic optical fiber into the light input/output of tees. Sulfide put in the bottom dish was liberated by addition of diluted acid through a port on the cover. The liberated hydrogen sulfide diffused into the porous membrane tube and reacted with alkaline nitroprusside acceptor in the tube. The color change in the long path porous membrane tube cell was real-time monitored in the transmission mode. The device responded linearly to sulfide concentration over the range of 0.5-150.0μmol/L with relative standard deviations less than 5% in all cases. The limits of detection for sulfide were within the range 0.2-1.5μmol/L in aqueous standard and newborn calf serum. The device was successfully applied to the determination of sulfide in human serum samples.
通过原位集成透气多孔管隔离和长程吸光度检测，制备了一种用于检测亚微摩尔级硫化物的经济高效且便携的装置。该装置由一对培养皿组成，其顶盖中有一个沿直径方向串连的多孔膜管。通过将丙烯酸光纤插入三通的光输入/输出端口，管的两端分别连接发光二极管和光电二极管。通过盖子上的端口加入稀酸，使置于底部培养皿中的硫化物释放出来。释放出的硫化氢扩散到多孔膜管中，并与管内的碱性硝普钠受体发生反应。以透射模式实时监测长程多孔膜管池中的颜色变化。该装置在0.5 - 150.0μmol/L范围内对硫化物浓度呈线性响应，所有情况下相对标准偏差均小于5%。在水标准溶液和新生小牛血清中，硫化物的检测限在0.2 - 1.5μmol/L范围内。该装置已成功应用于人体血清样品中硫化物的测定。
