Liu Changhui, Zheng Jing, Deng Li, Ma Cheng, Li Jishan, Li Yinhui, Yang Sheng, Yang Jinfeng, Wang Jing, Yang Ronghua
†State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
‡Department of Chemistry and Environmental Engineering, Hunan City University, Yiyang, 413000, P. R. China.
ACS Appl Mater Interfaces. 2015 Jun 10;7(22):11930-8. doi: 10.1021/acsami.5b01787. Epub 2015 May 29.
Targeting nanocontainers to the pathological zone and controlling release of their cargoes, in particular delivery of anticancer drugs to specific tumor cells in a targeted and controlled manner, remain the key challenges in drug delivery. This paper reports the development of a traceable and tumor-targeted intracellular drug release nanocontainer. The nanocontainer is obtained by in situ growth of silver nanoparticles (AgNPs) on the surfaces of mesoporous silica nanospheres (MSNs) using a DNA-templated process. Additionally, drug release from the nanopores is achieved by selective glutathione (GSH)-triggered dismantle of the AgNPs, and the concurrent fluorescence change allows real-time monitoring of drug release efficacy and facile visualization of in vivo delivery events. After being functionalized with sgc8 aptamer on the outer shell of the AgNPs, the targeted nanocontainers are delivered into acute lymphoblastic leukemia cells by aptamer-mediated recognition and endocytosis. Moreover, the GSH-responsive process presents an improvement in the cell-specific drug release and chemotherapeutic inhibition of tumor growth.
将纳米容器靶向至病理区域并控制其所载物质的释放，尤其是以靶向和可控方式将抗癌药物递送至特定肿瘤细胞，仍然是药物递送领域的关键挑战。本文报道了一种可追踪且靶向肿瘤的细胞内药物释放纳米容器的研发。该纳米容器是通过使用DNA模板法在介孔二氧化硅纳米球（MSN）表面原位生长银纳米颗粒（AgNP）而获得的。此外，纳米孔中的药物释放是通过谷胱甘肽（GSH）选择性触发AgNP的拆解来实现的，同时荧光变化允许实时监测药物释放效果并便于可视化体内递送过程。在用sgc8适配体对AgNP外壳进行功能化后，靶向纳米容器通过适配体介导的识别和内吞作用被递送至急性淋巴细胞白血病细胞中。此外，GSH响应过程在细胞特异性药物释放和肿瘤生长的化疗抑制方面有了改进。
