在嗜盐碱条件下，磁性纳米颗粒对固定化硫氧化细菌——嗜碱硫弧菌进行脱硫处理。
Desulfurization of immobilized sulfur-oxidizing bacteria, Thialkalivibrio versutus, by magnetic nanaoparticles under haloalkaliphilic conditions.
作者信息
Xu Xiaohui, Cai Yufeng, Song Ziyu, Qiu Xiaoling, Zhou Jiemin, Liu Yilan, Mu Tingzhen, Wu Dan, Guan Yueping, Xing Jianmin
机构信息
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
出版信息
Biotechnol Lett. 2015 Aug;37(8):1631-5. doi: 10.1007/s10529-015-1845-x. Epub 2015 May 13.
OBJECTIVES
As a haloalkaliphilic, sulfur-oxidizing bacteria, Thialkalivibrio versutus D301 can remove sulfide, thiosulfate and polysulfide in wastewater, we investigated how it might be reused when mixed with high concentrations of elemental sulfur.
RESULTS
A process is described to immobilize T. versutus cells by using superparamagnetic Fe3O4 nanoparticles (NPs) under haloalkaliphilic conditions (i.e. pH 9.5, 0.5 M Na(+)). The saturation magnetization value (δs) of immobilized cells was 55.1 emu/g. The Fe3O4 NPs-coated cells had the similar sulfur oxidization activity to that of free cells, and they could be reused six batch cycles. Analysis of hydraulic diameters showed that bacterial cells were immobilized by Fe3O4 NPs due to the nano-size effects.
CONCLUSIONS
Magnetic immobilization is a convenient technique for cell immobilization under haloalkaliphilic conditions and is a promising technology for large scale application.
目的
作为一种嗜盐碱、硫氧化细菌， Versutus硫碱弧菌D301能够去除废水中的硫化物、硫代硫酸盐和多硫化物，我们研究了将其与高浓度元素硫混合时如何实现再利用。
结果
描述了一种在嗜盐碱条件下（即pH 9.5、0.5 M Na(+)）使用超顺磁性Fe3O4纳米颗粒（NPs）固定Versutus硫碱弧菌细胞的方法。固定化细胞的饱和磁化强度值（δs）为55.1 emu/g。Fe3O4 NPs包被的细胞具有与游离细胞相似的硫氧化活性，并且可以重复使用六个批次循环。水力直径分析表明，由于纳米尺寸效应，细菌细胞被Fe3O4 NPs固定。
结论
磁性固定是一种在嗜盐碱条件下方便的细胞固定技术，是一种具有大规模应用前景的技术。
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