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用功能性磁性FeO纳米颗粒修饰的SY095的降解

Degradation of SY095 modified with functional magnetic FeO nanoparticles.

作者信息

Ma Xiaolei, Duan Duomo, Wang Xunliang, Cao Junrui, Qiu Jinquan, Xie Baolong

机构信息

Microbiology and Biotechnology Research Laboratory, The Institute of Seawater Desalination and Multi-Purpose Utilization, Ministry of Natural Resources of the People's Republic of China (MNR), Tianjin 300192, People's Republic of China.

Tianjin Rehabilitation Center, The PLA Joint Logistic Support Force, Tianjin, 300191, People's Republic of China.

出版信息

R Soc Open Sci. 2021 Dec 22;8(12):211172. doi: 10.1098/rsos.211172. eCollection 2021 Dec.

Abstract

Alkali-surfactant-polymer flooding technology is widely employed to extract crude oil to enhance its production. The bacterial strain SY095 has shown high degradation activity of alkane of crude oil. In the past, many treatment strategies have been implemented to reduce oil concentration in wastewater. Previous studies mainly focused on the extracellular products of rather than its degradation properties. In the current study, we designed an immobilization method to modify the surface of SY095 with functional FeO nanoparticles (NPs) for biodegradation of crude oil and separation of the immobilized bacteria after degradation. We characterize the synthesized NPs through various methods, including scanning electron microscope energy-dispersive spectrometer, Fourier transform infrared spectroscopy, X-ray diffraction (XRD) and a vibrating sample magnetometer. We found that the size of the synthesized NPs was approximately 100 nm. Our results showed that SY095 was successfully coated with functional magnetic NPs (MNPs) that could be easily separated from the solution via the application of an external magnetic field. The coated cells had a high tolerance for heavy metals. Our findings demonstrated that the immobilization of MNPs to bacterial surfaces is a promising approach for the degradation of crude oil.

摘要

碱-表面活性剂-聚合物驱油技术被广泛用于开采原油以提高产量。细菌菌株SY095对原油中的烷烃表现出高降解活性。过去,人们实施了许多处理策略来降低废水中的油浓度。以往的研究主要集中在其胞外产物而非降解特性上。在本研究中,我们设计了一种固定化方法,用功能性FeO纳米颗粒(NPs)修饰SY095的表面,用于原油生物降解以及降解后固定化细菌的分离。我们通过多种方法对合成的NPs进行表征,包括扫描电子显微镜能谱仪、傅里叶变换红外光谱、X射线衍射(XRD)和振动样品磁强计。我们发现合成的NPs尺寸约为100nm。我们的结果表明,SY095成功地被功能性磁性纳米颗粒(MNPs)包覆,通过施加外部磁场可以很容易地从溶液中分离出来。包覆的细胞对重金属具有高耐受性。我们的研究结果表明,将MNPs固定在细菌表面是一种很有前景的原油降解方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e66e/8692970/8b3780f5187e/rsos211172f01.jpg

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