Sayyed Danishmalik Rafiq, Nimse Satish Balasaheb, Song Keum-Soo, Sung Nackmoon, Kim Taisun
Institute for Applied Chemistry and Department of Chemistry, Hallym University, 1 Okcheon-dong, Chuncheon, 200-702, South Korea.
Anal Bioanal Chem. 2015 Jul;407(19):5739-45. doi: 10.1007/s00216-015-8754-7. Epub 2015 May 13.
The MTB-DR-RIF 9G membrane can detect by detecting multiple mutations in multiple codons. The MTB-DR-RIF 9G membrane possesses clinical applicability in point-of-care settings for the following reasons: (i) 100% similar results with that of the sequencing analysis for clinical samples, (ii) discrimination of the multiple mutations in multiple codons, (iii) a specific/non-specific hybridization ratio higher than 350:1, and (iv) the sensitivity was found to be 1-10 copies/test for detection and discrimination of the wild and mutant TB strains. Graphical abstract Schematic illustration of the effect of controller DNA on the hybridization of the immobilized probes (corresponding to the wild TB strain) with the PCR product of (a) wild TB strain and (b) mutant TB strain.
MTB-DR-RIF 9G膜可通过检测多个密码子中的多种突变来进行检测。MTB-DR-RIF 9G膜在即时检测环境中具有临床适用性，原因如下：（i）与临床样本测序分析结果的相似度达100%；（ii）可区分多个密码子中的多种突变；（iii）特异性/非特异性杂交率高于350:1；（iv）检测和区分野生型和突变型结核菌株的灵敏度为1-10拷贝/测试。图形摘要 控制器DNA对固定探针（对应野生型结核菌株）与（a）野生型结核菌株和（b）突变型结核菌株的PCR产物杂交影响的示意图。
