一种在特定位置含有共价、热可裂解链间交联的DNA双链体的简单、高产率合成方法。
A simple, high-yield synthesis of DNA duplexes containing a covalent, thermally cleavable interstrand cross-link at a defined location.
作者信息
Gamboa Varela Jacqueline, Gates Kent S
机构信息
Department of Chemistry, University of Missouri, Columbia, MO 65211 (USA).
Department of Biochemistry, University of Missouri, Columbia, MO 65211 (USA).
出版信息
Angew Chem Int Ed Engl. 2015 Jun 22;54(26):7666-9. doi: 10.1002/anie.201502566. Epub 2015 May 12.
Abstract
Interstrand DNA-DNA cross-links are highly toxic to cells because these lesions block the extraction of information from the genetic material. The pathways by which cells repair cross-links are important, but not well understood. The preparation of chemically well-defined cross-linked DNA substrates represents a significant challenge in the study of cross-link repair. Here a simple method is reported that employs "post-synthetic" modifications of commercially available 2'-deoxyoligonucleotides to install a single cross-link in high yield at a specified location within a DNA duplex. The cross-linking process exploits the formation of a hydrazone between a non-natural N(4) -amino-2'-deoxycytidine nucleobase and the aldehyde residue of an abasic site in duplex DNA. The resulting cross-link is stable under physiological conditions, but can be readily dissociated and re-formed through heating-cooling cycles.
摘要
链间DNA-DNA交联对细胞具有高度毒性，因为这些损伤会阻碍从遗传物质中提取信息。细胞修复交联的途径很重要，但尚未得到充分了解。在交联修复研究中，制备化学性质明确的交联DNA底物是一项重大挑战。本文报道了一种简单的方法，该方法利用市售2'-脱氧寡核苷酸的“合成后”修饰，在DNA双链体的特定位置以高产率安装单个交联。交联过程利用了非天然N(4)-氨基-2'-脱氧胞嘧啶核苷酸碱基与双链DNA中无碱基位点的醛残基之间腙的形成。所得交联在生理条件下稳定，但可通过加热-冷却循环轻易解离并重新形成。
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