含有鸟嘌呤残基与无碱基位点之间位点特异性、还原型、化学稳定的共价链间交联的寡脱氧核苷酸双链体的制备与纯化
Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically Stable Covalent Interstrand Cross-Link Between a Guanine Residue and an Abasic Site.
作者信息
Nejad Maryam Imani, Guo Xu, Housh Kurt, Nel Christopher, Yang Zhiyu, Price Nathan E, Wang Yinsheng, Gates Kent S
机构信息
Department of Chemistry, University of Missouri, Columbia, MO, USA.
Department of Chemistry, University of California Riverside, Riverside, CA, USA.
出版信息
Methods Mol Biol. 2019;1973:163-175. doi: 10.1007/978-1-4939-9216-4_10.
Abstract
Methods for the preparation of DNA duplexes containing interstrand covalent cross-links may facilitate research in the fields of biochemistry, molecular biology, nanotechnology, and materials science. Here we report methods for the synthesis and isolation of DNA duplexes containing a site-specific, chemically stable, reduced covalent interstrand cross-link between a guanine residue and an abasic site. The method uses experimental techniques and equipment that are common in most biochemical laboratories and inexpensive, commercially available oligonucleotides and reagents.
摘要
制备含有链间共价交联的DNA双链体的方法可能会促进生物化学、分子生物学、纳米技术和材料科学领域的研究。在此，我们报告了合成和分离含有鸟嘌呤残基与无碱基位点之间位点特异性、化学稳定、还原型共价链间交联的DNA双链体的方法。该方法使用了大多数生化实验室常见的实验技术和设备，以及廉价的、可商购的寡核苷酸和试剂。
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