Ji Bin, Wei Li, Chen Dan, Wang Hongyu, Li Zhenhua, Yang Kai
School of Civil Engineering, Wuhan University, Wuhan, 430072, China.
Appl Microbiol Biotechnol. 2015 Jul;99(13):5731-8. doi: 10.1007/s00253-015-6667-1. Epub 2015 May 13.
Biological treatment of domestic sewage low C/N ratio was accomplished in a pilot-scale sequencing batch biofilm filter (SBBF). The novel hybrid bioreactor consisted of bio-band in the upper and anthracite filter media in the bottom, which combined a sequencing batch biofilm reactor (SBBR) with a biological filter. The average removal efficiency values of chemical oxygen demand (COD), ammonium nitrogen (NH4 (+)-N), total nitrogen (TN), and total phosphorus (TP) were 89.4, 83.3, 62.9, and 48.7%, respectively. A 454-pyrosequencing technology was employed to investigate the microbial communities of the influent (J1) and the biofilm (J2) on the bio-band on day 40. Pyrosequencing analysis of the 16S rRNA gene revealed the community of the biofilm consisted of Gammaproteobacteria (48.6%), Planctomycetacia (18.0%), Alphaproteobacteria (13.7%), Clostridia (9.6%), Desulfonatronum (18.5%), Actinobacteria (1.9%), and Bacilli (1.7%), accounting for 93.6% of total operational taxonomic units at genera level. Acinetobacter tjernbergiae and Acinetobacter lwoffii were the most abundant species, suggesting that denitrifying phosphorus removal was achieved in the SBBF.
采用中试规模的序批式生物膜滤池（SBBF）对低C/N比的生活污水进行生物处理。这种新型复合生物反应器上部为生物带，底部为无烟煤滤料，它将序批式生物膜反应器（SBBR）与生物滤池结合在一起。化学需氧量（COD）、氨氮（NH4(+)-N）、总氮（TN）和总磷（TP）的平均去除效率分别为89.4%、83.3%、62.9%和48.7%。在第40天，采用454焦磷酸测序技术研究了进水（J1）和生物带上生物膜（J2）的微生物群落。对16S rRNA基因的焦磷酸测序分析表明，生物膜群落由γ-变形菌纲（48.6%）、浮霉菌纲（18.0%）、α-变形菌纲（13.7%）、梭菌纲（9.6%）、脱磺弧菌属（18.5%）、放线菌纲（1.9%）和芽孢杆菌纲（1.7%）组成，在属水平上占总操作分类单元的93.6%。tjernbergiae不动杆菌和鲁氏不动杆菌是最优势的物种，这表明SBBF实现了反硝化除磷。
