Department of Life Science, Graduate School, Kyonggi University, Suwon-si, Gyeonggi-do, Republic of Korea.
Department of Biological Engineering, Graduate School, Kyonggi University, Suwon-si, Gyeonggi-do, Republic of Korea.
PLoS One. 2020 May 19;15(5):e0233042. doi: 10.1371/journal.pone.0233042. eCollection 2020.
Effective biological treatment of marine wastewater is not well-known. Accumulation of nitrogen and phosphorus from land-based effluent is a crucial cause of red-tide in marine systems. The purpose of the study is to reduce nitrogen and phosphorus in marine wastewater with a pilot plant-scale sequencing batch reactor (SBR) system by using marine sediment as eco-friendly and effective biological materials, and elucidate which bacterial strains in sludge from marine sediment influence the performance of SBR. By applying eco-friendly high efficiency marine sludge (eco-HEMS), the treatment performance was 15 m3 d-1 of treatment amount in 4.5 m3 of the reactor with the average removal efficiency of 89.3% for total nitrogen and 94.9% for total phosphorus at the optimal operation condition in summer. Moreover, the average removal efficiency was 84.0% for total nitrogen and 88.3% for total phosphorus in winter although biological treatment efficiency in winter is generally lower due to bacterial lower activity. These results were revealed by the DNA barcoding analysis of 16s rRNA amplicon sequencing of samples from the sludge in winter. The comparative analysis of the bacterial community composition in sludge at the high efficiency of the system showed the predominant genera Psychromonas (significantly increased to 45.6% relative abundance), Vibrio (13.3%), Gaetbulibacter (5.7%), and Psychroserpens (4.3%) in the 4 week adaptation after adding marine sediment, suggesting that those predominant bacteria influenced the treatment performance in winter.
海洋废水的有效生物处理方法并不为人所知。陆基污水中氮磷的积累是海洋系统赤潮的一个关键原因。本研究的目的是利用海洋沉积物作为环保且有效的生物材料，通过中试规模序批式反应器（SBR）系统减少海洋废水中的氮和磷，并阐明海洋沉积物污泥中的哪些细菌菌株会影响 SBR 的性能。应用环保高效海洋污泥（eco-HEMS），在夏季最佳运行条件下，在 4.5 立方米的反应器中处理量为 15 立方米/天，总氮的平均去除效率为 89.3%，总磷的平均去除效率为 94.9%。此外，尽管冬季由于细菌活性较低，生物处理效率普遍较低，但冬季的平均去除效率仍为总氮 84.0%和总磷 88.3%。这些结果是通过冬季污泥样品的 16s rRNA 扩增子测序的 DNA 条形码分析揭示的。系统高效运行时污泥中细菌群落组成的对比分析表明，在添加海洋沉积物后 4 周的适应期内，优势属Psychromonas（相对丰度显著增加至 45.6%）、Vibrio（13.3%）、Gaetbulibacter（5.7%）和Psychroserpens（4.3%），表明这些优势细菌影响了冬季的处理性能。
