非小细胞肺癌患者中CD4+CD25+调节性T细胞与缺氧诱导因子-1α及Ki-67表达的关系
Relationship between CD4+CD25+ Treg and expression of HIF-1α and Ki-67 in NSCLC patients.
作者信息
He L-Y, Li L, Guo M-L, Zhang Y, Zhang H-Z
机构信息
People's Hospital of Hebei Province, Shijiazhuang, Hebei Province, China.
出版信息
Eur Rev Med Pharmacol Sci. 2015 Apr;19(8):1351-5.
OBJECTIVE
To investigate the relationship between the CD4+CD25+ Treg cell proportion in the peripheral blood and the clinicopathologic features of non-small cell lung cancer (NSCLC) patients and hypoxia-inducible factor-1α (HIF-1α), Ki-67 expression.
PATIENTS AND METHODS
Flow cytometry was used to measure the CD4+CD25+ Treg cell level in peripheral blood and immunohistochemical staining used to detect the expression of HIF-1α and Ki-67 protein in cancer tissue of each of 50 NSCLC patients.
RESULTS
The level of CD4+CD25+ Treg cell in peripheral blood was related to pathologic grades (t = 3.265, p = 0.006) and clinical stage (t = 4.417, p = 0. 001) of NSCLC instead of to patient's gender and pathologic type of tumor (p > 0.05). The level of CD4+CD25+ Treg cell was positively correlated with the expression of HIF-1α (r = 0.711, p = 0.003) and Ki-67 (r = 0.517, p = 0.04), respectively.
CONCLUSIONS
CD4+CD25+ Treg cell can be used as a predictor of immune status and prognosis of NSCLC patients and the levels of HIF-1α and Ki-67 protein expression may relate to inhibition of immune cells.
目的
探讨非小细胞肺癌（NSCLC）患者外周血中CD4+CD25+调节性T细胞（Treg细胞）比例与临床病理特征以及缺氧诱导因子-1α（HIF-1α）、Ki-67表达之间的关系。
患者与方法
采用流式细胞术检测50例NSCLC患者外周血中CD4+CD25+ Treg细胞水平，采用免疫组织化学染色法检测癌组织中HIF-1α和Ki-67蛋白的表达。
结果
外周血中CD4+CD25+ Treg细胞水平与NSCLC的病理分级（t = 3.265，p = 0.006）和临床分期（t = 4.417，p = 0.001）有关，而与患者性别和肿瘤病理类型无关（p > 0.05）。CD4+CD25+ Treg细胞水平分别与HIF-1α表达（r = 0.711，p = 0.003）和Ki-67表达（r = 0.517，p = 0.04）呈正相关。
结论
CD4+CD25+ Treg细胞可作为NSCLC患者免疫状态和预后的预测指标，HIF-1α和Ki-67蛋白表达水平可能与免疫细胞抑制有关。
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