Altunkaynak M E, Akgül N, Yahyazadeh A, Altunkaynak B Z, Türkmen A P, Akgül H M, Aksak S, Ünal B
a Department of Histology and Embryology , Faculty of Medicine, Ondokuz Mayıs University , Samsun , Turkey.
b Department of Restorative Dentistry , Faculty of Dentistry, Atatürk University , Erzurum , Turkey.
Biotech Histochem. 2015;90(7):529-34. doi: 10.3109/10520295.2015.1024739. Epub 2015 May 13.
Mercury is ubiquitous in the environment; it is an occupational pollutant and a potential toxicant. We investigated the effects of exposure of rat testes to mercury vapor (Hg(0)). Twelve male rats were divided into two groups of six: the rats of the Hg(0) group were exposed to mercury (1 mg/m(3)/day) in a chamber for six weeks; the control group rats were housed under the same conditions without exposure to Hg(0). After the experimental period, the testes were removed, sections of testis were evaluated histopathologically after hematoxylin and eosin staining, and stereologically using the Cavalieri principle and optical fractionator methods. We found significant decreases in the total volume of testis, diameters of seminiferous tubules and total volume of seminiferous tubules. Significant decreases were detected in the numbers of Sertoli cells, spermatogonia, spermatocytes and spermatids of the Hg(0) group compared to the control group. In the Hg(0) exposed group, spermatogenic cells were degenerated and seminiferous tubules were atrophied.
汞在环境中无处不在；它是一种职业污染物和潜在的有毒物质。我们研究了大鼠睾丸暴露于汞蒸气（Hg(0)）的影响。将12只雄性大鼠分为两组，每组6只：Hg(0)组的大鼠在一个舱室内暴露于汞（1毫克/立方米/天）六周；对照组大鼠在相同条件下饲养，不暴露于Hg(0)。实验期结束后，取出睾丸，用苏木精和伊红染色后对睾丸切片进行组织病理学评估，并使用卡瓦列里原理和光学分割法进行体视学评估。我们发现睾丸总体积、生精小管直径和生精小管总体积显著减少。与对照组相比，Hg(0)组的支持细胞、精原细胞、精母细胞和精子细胞数量显著减少。在Hg(0)暴露组中，生精细胞退化，生精小管萎缩。
