Tenjin T, Kimura T, Takeda H, Sakagami S, Takahashi N, Shoji T, Asano G
2nd Dept. of Surgery, Nippon Medical School.
Gan To Kagaku Ryoho. 1989 Dec;16(12):3781-6.
We studied the effect of adriamycin on DNA synthesizing cells from the view point of cell cycle. DNA synthesizing cells were marked by bromodeoxyuridine, which is a known marker for such cells, before administration of adriamycin. Then, the level of this marker was sequentially measured by flow cytometry. At the low concentration (0.01 micrograms/ml), adriamycin caused delay of the shifting time to the S phase 16 hours after administration. The marked cells were accumulated at the G2M phase at a moderate concentration (0.1 micrograms/ml), and blocked at the S phase at the higher concentration (1.0 micrograms/ml).
我们从细胞周期的角度研究了阿霉素对DNA合成细胞的影响。在给予阿霉素之前，用溴脱氧尿苷标记DNA合成细胞，溴脱氧尿苷是这类细胞的一种已知标记物。然后，通过流式细胞术依次测量该标记物的水平。在低浓度（0.01微克/毫升）时，阿霉素导致给药后16小时进入S期的转移时间延迟。在中等浓度（0.1微克/毫升）时，标记细胞在G2M期积累，在较高浓度（1.0微克/毫升）时则阻滞在S期。
