Otón Joaquín, Sorzano Carlos Oscar S, Marabini Roberto, Pereiro Eva, Carazo Jose M
Opt Express. 2015 Apr 20;23(8):9567-72. doi: 10.1364/OE.23.009567.
Soft X-ray tomography (SXT) is becoming a powerful imaging technique to analyze eukaryotic whole cells close to their native state. Central to the analysis of the quality of SXT 3D reconstruction is the estimation of the spatial resolution and Depth of Field of the X-ray microscope. In turn, the characterization of the Modulation Transfer Function (MTF) of the optical system is key to calculate both parameters. Consequently, in this work we introduce a fully automated technique to accurately estimate the transfer function of such an optical system. Our proposal is based on the preprocessing of the experimental images to obtain an estimate of the input pattern, followed by the analysis in Fourier space of multiple orders of a Siemens Star test sample, extending in this way its measured frequency range.
软X射线断层扫描（SXT）正成为一种强大的成像技术，用于分析接近其天然状态的真核全细胞。SXT三维重建质量分析的核心是X射线显微镜空间分辨率和景深的估计。反过来，光学系统调制传递函数（MTF）的表征是计算这两个参数的关键。因此，在这项工作中，我们引入了一种全自动技术来准确估计这种光学系统的传递函数。我们的方法基于对实验图像的预处理以获得输入图案的估计，然后在傅里叶空间中分析西门子星测试样品的多个阶次，从而扩展其测量频率范围。
