Otón Joaquín, Pereiro Eva, Pérez-Berná Ana J, Millach Laia, Sorzano Carlos Oscar S, Marabini Roberto, Carazo José M
Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049, Madrid, Spain.
ALBA Synchrotron Light Source, Cerdanyola del Vallès, 08290, Barcelona, Spain.
Biomed Opt Express. 2016 Nov 14;7(12):5092-5103. doi: 10.1364/BOE.7.005092. eCollection 2016 Dec 1.
Full field soft X-ray microscopy is becoming a powerful imaging technique to analyze whole cells preserved under cryo conditions. Images obtained in these X-ray microscopes can be combined by tomographic reconstruction to quantitatively estimate the three-dimensional (3D) distribution of absorption coefficients inside the cell. The impulse response of an imaging system is one of the factors that limits the quality of the X-ray microscope reconstructions. The main goal of this work is to experimentally measure the 3D impulse response and to assess the optical resolution and depth of field of the Mistral microscope at ALBA synchrotron (Barcelona, Spain). To this end we measure the microscope apparent transfer function (ATF) and we use it to design a deblurring Wiener filter, obtaining an increase in the image quality when applied to experimental datasets collected at ALBA.
全场软X射线显微镜正成为一种强大的成像技术，用于分析在低温条件下保存的完整细胞。在这些X射线显微镜中获得的图像可以通过断层重建进行组合，以定量估计细胞内吸收系数的三维（3D）分布。成像系统的脉冲响应是限制X射线显微镜重建质量的因素之一。这项工作的主要目标是通过实验测量三维脉冲响应，并评估位于西班牙巴塞罗那阿尔巴同步加速器的米斯特拉尔显微镜的光学分辨率和景深。为此，我们测量了显微镜的表观传递函数（ATF），并使用它设计了一种去模糊维纳滤波器，将其应用于在阿尔巴收集的实验数据集时，图像质量得到了提高。
