Gore-Langton Jonathan K, Flax Shaun M, Pomfrey Rebecca L, Wetzell B Bradley, Riley Anthony L
Psychopharmacology Laboratory, Department of Psychology, American University, Washington, DC 20016, United States.
Psychopharmacology Laboratory, Department of Psychology, American University, Washington, DC 20016, United States.
Pharmacol Biochem Behav. 2015 Jul;134:99-105. doi: 10.1016/j.pbb.2015.05.002. Epub 2015 May 9.
The present experiments directly compared the ability of the conditioned taste and place aversion designs (CTA and CPA, respectively) to measure the aversive effects of lithium chloride (LiCl) in male Sprague-Dawley rats. In the CTA assessment (Experiment 1), rats were given one of two novel tastes paired with LiCl (0, 0.18, 0.32, 0.56 or 1mEq/kg) and the alternate novel taste paired with vehicle the next day. This was repeated three times, followed by a final two-bottle test. In the CPA assessment (Experiment 2), rats were given LiCl at the same doses and placed on one side of an unbiased two-chambered apparatus, followed by vehicle injection and placement on the opposite side on alternating days. This was repeated three times followed by free access to both sides in an assessment of relative preference. LiCl induced robust, dose-dependent taste aversions with rats receiving 0.32mEq/kg or greater consuming a smaller percentage of the drug-paired taste than that of controls. LiCl did not induce place aversions at any dose with LiCl- and vehicle-treated subjects displaying comparable preferences for the drug-paired side. The basis for the differences of the two designs in indexing LiCl's aversive effects was discussed.
本实验直接比较了条件性味觉厌恶设计（CTA）和条件性位置厌恶设计（CPA）分别测量氯化锂（LiCl）对雄性斯普拉格-道利大鼠厌恶效应的能力。在CTA评估（实验1）中，大鼠被给予两种新口味之一，并与LiCl（0、0.18、0.32、0.56或1mEq/kg）配对，另一种新口味则在第二天与赋形剂配对。此过程重复三次，随后进行最后的双瓶测试。在CPA评估（实验2）中，大鼠接受相同剂量的LiCl，并被放置在无偏倚的双室装置的一侧，随后交替日进行赋形剂注射并放置在另一侧。此过程重复三次，随后在相对偏好评估中让大鼠自由进入两侧。LiCl诱导了强烈的、剂量依赖性的味觉厌恶，接受0.32mEq/kg或更高剂量LiCl的大鼠摄入的与药物配对口味的比例低于对照组。LiCl在任何剂量下均未诱导位置厌恶，接受LiCl和赋形剂处理的大鼠对与药物配对一侧的偏好相当。文中讨论了两种设计在索引LiCl厌恶效应方面存在差异的原因。
