绿针假单胞菌金色亚种UCM B-306脂多糖O-多糖的结构

Structure of the O-polysaccharide of the lipopolysaccharide of Pseudomonas chlororaphis subsp. aureofaciens UCM B-306.

作者信息

Zdorovenko Evelina L, Varbanets Liudmyla D, Shashkov Alexander S, Kiprianova Elena A, Knirel Yuriy A

机构信息

N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.

D.K. Zabolotny Insitute of Microbiology and Virology, National Academy of Sciences, ul. Zabolotnogo 154, 03143 Kiev, Ukraine.

出版信息

Carbohydr Res. 2015 Jun 17;410:47-50. doi: 10.1016/j.carres.2015.03.019. Epub 2015 Apr 11.

DOI:10.1016/j.carres.2015.03.019

PMID:25969402

Abstract

Structure of the O-specific polysaccharide from Pseudomonas chlororaphis subsp. aureofaciens UCM B-306 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The polysaccharide is built up of trisaccharide repeats containing D-rhamnose, 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-QuiNAc4NAc), and 2-acetamido-2-deoxy-D-galacturonic acid (D-GalNAcA), which is amidated in ∼40% repeats. It was suggested that the O-polysaccharide has a blockwise structure, which can be presented as follows: -[→3)-α-D-Rhap-(1→4)-α-D-GalpNAcA-(1→3)-α-D-QuipNAc4NAc-(1-]n→ and -[→3)-α-D-Rhap-(1→4)-α-D-GalpNAcAN-(1→3)-α-D-QuipNAc4NAc-(1-]m→, where GalNAcAN indicates 2-acetamido-2-deoxy-D-galacturonamide, n:m=∼3:2.

摘要

通过糖分析以及一维和二维（1）H和（13）C NMR光谱对绿针假单胞菌金色亚种UCM B - 306的O - 特异性多糖结构进行了阐明。该多糖由含有D - 鼠李糖、2,4 - 二乙酰氨基 - 2,4,6 - 三脱氧 - D - 葡萄糖（D - QuiNAc4NAc）和2 - 乙酰氨基 - 2 - 脱氧 - D - 半乳糖醛酸（D - GalNAcA）的三糖重复单元组成，其中约40%的重复单元中的D - GalNAcA发生了酰胺化。有人提出该O - 多糖具有嵌段结构，可表示如下：-[→3)-α - D - Rhap-(1→4)-α - D - GalpNAcA-(1→3)-α - D - QuipNAc4NAc-(1-]n→ 和 -[→3)-α - D - Rhap-(1→4)-α - D - GalpNAcAN-(1→3)-α - D - QuipNAc4NAc-(1-]m→，其中GalNAcAN表示2 - 乙酰氨基 - 2 - 脱氧 - D - 半乳糖酰胺，n:m = ∼3:2。