Gu Hui, Varner Erika L, Groskreutz Stephen R, Michael Adrian C, Weber Stephen G
#Department of Chemistry, East China Normal University, Shanghai 200062, PRC.
†Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
Anal Chem. 2015 Jun 16;87(12):6088-94. doi: 10.1021/acs.analchem.5b00633. Epub 2015 May 26.
Microdialysis is often applied to understanding brain function. Because neurotransmission involves rapid events, increasing the temporal resolution of in vivo measurements is desirable. Here, we demonstrate microdialysis with online capillary liquid chromatography for the analysis of 1 min rat brain dialysate samples at 1 min intervals. Mobile phase optimization involved adjusting the pH, buffer composition, and surfactant concentration to eliminate interferences with the dopamine peak. By analyzing electrically evoked dopamine transients carefully synchronized with the switching of the online LC sample valve, we demonstrate that our system has both 1 min sampling capabilities and bona fide 1 min temporal resolution. Evoked DA transients were confined to single, 1 min brain dialysate samples. After uptake inhibition with nomifensine (20 mg/kg i.p.), responses to electrical stimuli of 1 s duration were detected.
微透析常用于理解脑功能。由于神经传递涉及快速事件，提高体内测量的时间分辨率是很有必要的。在此，我们展示了在线毛细管液相色谱微透析技术，用于每隔1分钟分析大鼠脑透析液样本。流动相优化包括调节pH值、缓冲液组成和表面活性剂浓度，以消除对多巴胺峰的干扰。通过仔细分析与在线液相色谱样品阀切换精确同步的电诱发多巴胺瞬变，我们证明我们的系统具有1分钟采样能力和真正的1分钟时间分辨率。诱发的多巴胺瞬变局限于单个1分钟的脑透析液样本。在用诺米芬辛（腹腔注射20mg/kg）抑制摄取后，检测到对持续1秒电刺激的反应。
