Biodiversity Centre, Institut de Recherche en Biologie Végétale, Université de Montréal and Jardin botanique de Montréal, Montréal, QC, Canada.
Département de Géographie, Université de Montréal, Montréal, QC, Canada.
Environ Microbiol. 2015 Aug;17(8):3025-38. doi: 10.1111/1462-2920.12900. Epub 2015 Jun 19.
Although plants introduced for site restoration are pre-selected for specific traits (e.g. trace element bioaccumulation, rapid growth in poor soils), the in situ success of these plants likely depends on the recruitment of appropriate rhizosphere microorganisms from their new environment. We introduced three willow (Salix spp.) cultivars to a contaminated landfill, and performed soil chemical analyses, plant measurements, and Ion Torrent sequencing of rhizospheric fungal and bacterial communities at 4 and 16 months post-planting. The abundance of certain dominant fungi was linked to willow accumulation of Zn, the most abundant trace element at the site. Interestingly, total Zn accumulation was better explained by fungal community structure 4 months post-planting than 16 months post-planting, suggesting that initial microbial recruitment may be critical. In addition, when the putative ectomycorrhizal fungi Sphaerosporella brunnea and Inocybe sp. dominated the rhizosphere 4 months post-planting, Zn accumulation efficiency was negatively correlated with fungal diversity. Although field studies such as this rely on correlation, these results suggest that the soil microbiome may have the greatest impact on plant function during the early stages of growth, and that plant-fungus specificity may be essential.
虽然用于场地修复的植物是根据特定特性（例如微量元素生物积累、贫瘠土壤中的快速生长）预先选择的，但这些植物在现场的成功可能取决于从新环境中招募适当的根际微生物。我们将三种柳树（柳属）品种引入受污染的垃圾填埋场，并在种植后 4 个月和 16 个月进行土壤化学分析、植物测量以及根际真菌和细菌群落的 Ion Torrent 测序。某些优势真菌的丰度与柳树对锌（该地点最丰富的微量元素）的积累有关。有趣的是，种植后 4 个月真菌群落结构对总锌积累的解释要好于种植后 16 个月，这表明初始微生物的招募可能至关重要。此外，当假定的外生菌根真菌 Sphaerosporella brunnea 和 Inocybe sp. 在种植后 4 个月主导根际时，锌积累效率与真菌多样性呈负相关。尽管此类田间研究依赖于相关性，但这些结果表明，在生长的早期阶段，土壤微生物组可能对植物功能的影响最大，并且植物-真菌特异性可能是必不可少的。
