Balmayor Elizabeth R, Baran Turker E, Unger Marina, Marques Alexandra P, Azevedo Helena S, Reis Rui L
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Taipas, Guimarães, Portugal.
IBB - Institute for Biotechnology and Bioengineering, PT Associated Laboratory, AvePark, 4806-909 Taipas, Guimarães, Portugal.
J Biomed Mater Res B Appl Biomater. 2015 Nov;103(8):1610-20. doi: 10.1002/jbm.b.33343. Epub 2014 Dec 30.
The effect of α-amylase degradation on the release of gentamicin from starch-conjugated chitosan microparticles was investigated up to 60 days. Scanning electron microscopic observations showed an increase in the porosity and surface roughness of the microparticles as well as reduced diameters. This was confirmed by 67% weight loss of the microparticles in the presence of α-amylase. Over time, a highly porous matrix was obtained leading to increased permeability and increased water uptake with possible diffusion of gentamicin. Indeed, a faster release of gentamicin was observed with α-amylase. Starch-conjugated chitosan particles are non-toxic and highly biocompatible for an osteoblast (SaOs-2) and fibroblast (L929) cell line as well as adipose-derived stem cells. When differently produced starch-conjugated chitosan particles were tested, their cytotoxic effect on SaOs-2 cells was found to be dependent on the crosslinking agent and on the amount of starch used.
研究了α-淀粉酶降解对庆大霉素从淀粉共轭壳聚糖微粒中释放的影响，时间长达60天。扫描电子显微镜观察显示，微粒的孔隙率和表面粗糙度增加，直径减小。在α-淀粉酶存在的情况下，微粒重量损失67%，证实了这一点。随着时间的推移，获得了高度多孔的基质，导致渗透性增加和吸水量增加，庆大霉素可能扩散。事实上，在α-淀粉酶作用下，观察到庆大霉素释放更快。淀粉共轭壳聚糖颗粒对成骨细胞（SaOs-2）、成纤维细胞（L929）细胞系以及脂肪来源干细胞无毒且具有高度生物相容性。当测试不同生产方式的淀粉共轭壳聚糖颗粒时，发现它们对SaOs-2细胞的细胞毒性作用取决于交联剂和所用淀粉的量。
