Balmayor E R, Baran E T, Azevedo H S, Reis R L
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal(1); IBB - Institute for Biotechnology and Bioengineering, PT Associated Laboratory, Guimarães, Portugal(2).
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal(1); IBB - Institute for Biotechnology and Bioengineering, PT Associated Laboratory, Guimarães, Portugal(2).
Carbohydr Polym. 2012 Jan 4;87(1):32-39. doi: 10.1016/j.carbpol.2011.06.078. Epub 2011 Jul 7.
Starch-conjugated chitosan microparticles were produced aimed to be used as a carrier for the long term sustained/controlled release of antibiotic drugs to control bone infection. The microparticles were prepared by a reductive alkylation crosslinking method. The obtained microparticles showed a spherical shape, with a slightly rough and porous surface, and a size range of 80-150μm. Gentamicin was entrapped into the starch-conjugated chitosan microparticles and its release profile was studied in vitro. Increasing concentrations of gentamicin (from 50 to 150mg/mL) led to a decrease in the encapsulation efficiency (from 67 to 55%), while drug loading increased from 4 to 27%. A sustained release of gentamicin was observed over a period of 30 days. The release kinetics could be controlled using an ionic crosslinker agent. In addition, a bacterial inhibition test on Staphylococcus aureus shows a diameter of the sample inhibition zone ranging from 12 to 17mm (70-100% of relative activity).
制备淀粉共轭壳聚糖微粒的目的是将其用作载体，用于抗生素药物的长期持续/控释，以控制骨感染。通过还原烷基化交联法制备微粒。所得微粒呈球形，表面略粗糙且多孔，尺寸范围为80 - 150μm。将庆大霉素包封于淀粉共轭壳聚糖微粒中，并对其体外释放曲线进行了研究。庆大霉素浓度增加（从50mg/mL至150mg/mL）导致包封效率降低（从67%降至55%），而载药量从4%增加至27%。在30天的时间内观察到庆大霉素的持续释放。使用离子交联剂可控制释放动力学。此外，对金黄色葡萄球菌的抑菌试验表明，样品抑菌圈直径范围为12至17mm（相对活性为70 - 100%）。
