Fujihara S, Harada Y
Department of Natural Resources, National Institute of Agro-Environmental Sciences, Tsukuba, Japan.
Biochem Biophys Res Commun. 1989 Dec 15;165(2):659-66. doi: 10.1016/s0006-291x(89)80016-6.
Polyamines in various root nodule bacteria including Bradyrhizobium japonicum, Rhizobium fredii, R. leguminosarum, R. meliloti and R. loti were identified by capillary gas chromatography. Homospermidine was the polyamine present in highest concentration in all the rhizobia tested. In addition to putrescine and homospermidine, fast-growing type of rhizobial cells contained a novel polyamine, aminobutylhomospermidine, NH2(CH2)4NH(CH2)4NH(CH2)4NH2. The unusual tetraamine was not found in the cells of slow-growing type of rhizobia throughout their growth period, indicating a difference in polyamine metabolism between fast-growing type and slow-growing type of root nodule bacteria.
通过毛细管气相色谱法鉴定了包括日本慢生根瘤菌、费氏中华根瘤菌、豌豆根瘤菌、苜蓿根瘤菌和百脉根根瘤菌在内的各种根瘤菌中的多胺。高亚精胺是所有测试根瘤菌中含量最高的多胺。除腐胺和高亚精胺外，快速生长型根瘤菌细胞还含有一种新型多胺，氨基丁基高亚精胺，NH2(CH2)4NH(CH2)4NH(CH2)4NH2。在整个生长期间，在慢速生长型根瘤菌细胞中未发现这种异常的四胺，这表明快速生长型和慢速生长型根瘤菌之间多胺代谢存在差异。
