Guo Ying, Zhao Panpan, Guo Guangyang, Hu Zhibo, Tian Li, Zhang Kexin, Zhang Wen, Xing Mingwei
College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, Heilongjiang Province, China.
Biol Trace Elem Res. 2015 Dec;168(2):490-9. doi: 10.1007/s12011-015-0357-9. Epub 2015 May 14.
Arsenic (As) is a widely distributed trace element which is known to be associated with numerous adverse effects on human beings and animals. Arsenic trioxide (As2O3) is an inorganic arsenical-containing toxic compound. The effect of excessive amounts of As2O3 exposure on gastrointestinal tract tissue damage in cocks is still unknown. This study was conducted to investigate the effect of As2O3 exposure on gastrointestinal tract tissue damage in cocks. In total, 72 1-day-old male Hyline cocks were randomly divided into four groups and fed either a commercial diet or an As2O3 supplement diet containing 7.5, 15, and 30 mg/kg As2O3. The experiment lasted for 90 days and gastrointestinal tract tissue samples (gizzard, glandular stomach, duodenum, jejunum, ileum, cecum, and rectum) were collected at 30, 60, and 90 days. Catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) activities; malondialdehyde (MDA) contents; and hydroxyl radical (OH·)-mediated inhibition were examined. Furthermore, the results demonstrated that MDA content in the gastrointestinal tract was increased, while the activities of CAT, GSH, and GSH-Px and the ability to resist OH· was decreased in the As2O3 treatment groups. Extensive damage was observed in the gastrointestinal tract. These findings indicated that As2O3 exposure caused oxidative damage in the gastrointestinal tract of cocks due to alterations in antioxidant enzyme activities and elevation of free radicals.
砷（As）是一种广泛分布的微量元素，已知会对人类和动物产生多种不良影响。三氧化二砷（As2O3）是一种含无机砷的有毒化合物。过量暴露于As2O3对公鸡胃肠道组织损伤的影响尚不清楚。本研究旨在调查暴露于As2O3对公鸡胃肠道组织损伤的影响。总共72只1日龄海兰雄性公鸡被随机分为四组，分别喂食商业饲料或含7.5、15和30mg/kg As2O3的As2O3补充饲料。实验持续90天，并在第30、60和90天收集胃肠道组织样本（肌胃、腺胃、十二指肠、空肠、回肠、盲肠和直肠）。检测了过氧化氢酶（CAT）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）的活性；丙二醛（MDA）含量；以及羟基自由基（OH·）介导的抑制作用。此外，结果表明，As2O3处理组胃肠道中MDA含量增加，而CAT、GSH和GSH-Px的活性以及抵抗OH·的能力降低。在胃肠道中观察到广泛的损伤。这些发现表明，由于抗氧化酶活性的改变和自由基的升高，暴露于As2O3会导致公鸡胃肠道的氧化损伤。
