Zhao Panpan, Zhang Kexin, Guo Guangyang, Sun Xiao, Chai Hongliang, Zhang Wen, Xing Mingwei
College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang Province, 150040, China.
Biol Trace Elem Res. 2016 Mar;170(1):224-36. doi: 10.1007/s12011-015-0462-9. Epub 2015 Aug 11.
Arsenic (As) is widely distributed in our living environment and is useful for industry, agriculture, medical treatment, and other fields. Arsenic trioxide (As2O3) is an existing form of As. Exposure to As2O3 has a toxic effect on humans and animals. It not only leads to skin cancer, peripheral vascular disease, hyperkeratosis, etc. but also interferes with the functioning of the gastrointestinal tract. The gastrointestinal tract is an important organ for animals to transform the food they eat into the nutrients their body needs for maintenance and growth. Heat shock proteins (Hsps) exist in the non-stress normal cells and their expression increases under stimuli. Therefore, we wonder whether the "stimulus" of As2O3 could change the messenger RNA (mRNA) abundance and expression level of Hsps in the gastrointestinal tract of birds. To investigate the relation between arseniasis and Hsp alterations in the chicken's gastrointestinal tract induced by an As2O3-supplemented diet, we selected 72 one-day-old male Hy-line chickens and randomly divided them into four groups. They were fed either a commercial diet or an As2O3-supplemented diet containing 7.5, 15, and 30 mg/kg As2O3. The experiment lasted for 90 days, and gastrointestinal tract tissue samples (gizzard, glandular stomach, duodenum, jejunum, ileum, cecum, and rectum) were collected at 30, 60, and 90 days. The mRNA contents of Hsps (including Hsp27, Hsp40, Hsp60, Hsp70, and Hsp90) were examined by real-time PCR (RT-PCR). The correlation between As2O3 and Hsp genes was assessed. In addition, the protein expression levels of Hsp60 and Hsp70 in the gastrointestinal tract tissue samples were measured by western blot. The results indicated that the mRNA expression levels and the Hsp expression levels in the gastrointestinal tract tissues of chickens with As2O3 supplementation increased at different time points in a dose-dependent manner (p < 0.05 or p < 0.01). These data suggested that arseniasis influenced the mRNA abundance of Hsp27, Hsp40, Hsp60, Hsp70, and Hsp90 and the protein expression levels of Hsp60 and Hsp70 in the chicken's gastrointestinal tract tissues.
砷（As）广泛分布于我们的生活环境中，在工业、农业、医疗等领域都有应用。三氧化二砷（As2O3）是砷的一种存在形式。接触As2O3对人和动物具有毒性作用。它不仅会导致皮肤癌、外周血管疾病、角化过度等，还会干扰胃肠道的功能。胃肠道是动物将所吃食物转化为身体维持和生长所需营养物质的重要器官。热休克蛋白（Hsps）存在于非应激的正常细胞中，在受到刺激时其表达会增加。因此，我们想知道As2O3的“刺激”是否会改变鸟类胃肠道中热休克蛋白的信使核糖核酸（mRNA）丰度和表达水平。为了研究在添加As2O3的日粮诱导下鸡胃肠道中砷中毒与热休克蛋白变化之间的关系，我们选取了72只1日龄的海兰褐公鸡，并将它们随机分为四组。分别给它们饲喂商品日粮或添加了7.5、15和30毫克/千克As2O3的日粮。实验持续90天，在第30、60和90天采集胃肠道组织样本（肌胃、腺胃、十二指肠、空肠、回肠、盲肠和直肠）。通过实时荧光定量聚合酶链反应（RT-PCR）检测热休克蛋白（包括Hsp27、Hsp40、Hsp60、Hsp70和Hsp90）的mRNA含量。评估As2O3与热休克蛋白基因之间的相关性。此外，通过蛋白质免疫印迹法检测胃肠道组织样本中Hsp60和Hsp70的蛋白表达水平。结果表明，添加As2O3的鸡胃肠道组织中，热休克蛋白的mRNA表达水平和Hsp表达水平在不同时间点呈剂量依赖性增加（p < 0.05或p < 0.01）。这些数据表明，砷中毒影响了鸡胃肠道组织中Hsp27、Hsp40、Hsp60、Hsp70和Hsp90的mRNA丰度以及Hsp60和Hsp70的蛋白表达水平。
