Roussan Dergham Ahmad, Khawaldeh Ghassan, Shaheen Ibrahim Ali
Provimi (a Cargill Company), PO Box 499, Amman 11118, Jordan
Provimi (a Cargill Company), PO Box 499, Amman 11118, Jordan.
Poult Sci. 2015 Jul;94(7):1499-503. doi: 10.3382/ps/pev119. Epub 2015 May 12.
Commercial chickens in Jordan suffer from respiratory disease of undetermined etiology. This study was designed to document the involvement of avian influenza virus (AIV) H9 subtype, Mycoplasma gallisepticum (MG), and Mycoplasma synoviae (MS) in this respiratory disease. In this study, trachea swabs from 350 commercial broiler chicken flocks that suffered from respiratory disease were tested for AIV H9 subtype by using reverse transcription (RT)-PCR and for MG and MS by using PCR. PCR and RT-PCR results showed that 23.7, 8.9, and 6.6% of these flocks were infected with AIV H9 subtype, MS, and MG, respectively, whereas 12.9 and 5.7% of these flocks were infected with both AIV H9 subtype and MS and AIV H9 subtype and MS, respectively. Furthermore, 42.3% of these flocks were negative for the above mentioned respiratory diseases. Further epidemiological studies are recommended to determine risk factors and evaluate the economic consequences of AIV H9 subtype, MG, and MS infections in the region. Furthermore, studies are required to isolate AIV H9 subtype, MG, and MS and develop vaccines against the local field isolates.
约旦的商业养殖鸡患有病因不明的呼吸道疾病。本研究旨在记录禽流感病毒（AIV）H9亚型、鸡毒支原体（MG）和滑膜支原体（MS）与这种呼吸道疾病的关联。在本研究中，对350个患有呼吸道疾病的商业肉鸡群的气管拭子进行检测，采用逆转录（RT）-PCR检测AIV H9亚型，采用PCR检测MG和MS。PCR和RT-PCR结果显示，这些鸡群中分别有23.7%、8.9%和6.6%感染了AIV H9亚型、MS和MG，而这些鸡群中分别有12.9%和5.7%同时感染了AIV H9亚型和MS以及AIV H9亚型和MG。此外，这些鸡群中有42.3%对上述呼吸道疾病检测呈阴性。建议进一步开展流行病学研究，以确定风险因素，并评估该地区AIV H9亚型、MG和MS感染的经济后果。此外，还需要开展研究以分离AIV H9亚型、MG和MS，并研发针对当地田间分离株的疫苗。
