Tobimatsu Hiroaki, Nishibuchi Yuichiro, Sudo Ryo, Goto Shinya, Tanishita Kazuo
Department of System Design Engineering, Keio University.
J Atheroscler Thromb. 2015;22(10):1091-9. doi: 10.5551/jat.28423. Epub 2015 May 13.
von Willebrand factor (VWF) plays an important role in the regulation of hemostasis and thrombosis formation, particularly under a high shear rate. However, the adhesive force due to the molecular interaction between VWF and glycoprotein Ibα (GPIbα) has not been fully explored. Thus, we employed atomic force microscopy to directly measure the adhesive force between VWF and GPIbα.
We measured the adhesive force between VWF and GPIbα at the molecular level using an atomic force microscope (AFM). An AFM cantilever was coated with recombinant N-terminus VWF binding site of GPIbα, whereas a cover glass was coated with native VWF.
The adhesive force at the molecular level was measured using an AFM. In the presence of 1 μg/mL VWF, the adhesion force was nearly 200 pN. As per the Gaussian fit analysis, the adhesive force of a single bond could have been 54 or 107 pN.
Our consideration with the Gaussian fit analysis proposed that the adhesive force of a single bond could be 54 pN, which is very close to that obtained by optical tweezers (50 pN).
血管性血友病因子（VWF）在止血和血栓形成的调节中起重要作用，尤其是在高剪切速率下。然而，VWF与糖蛋白Ibα（GPIbα）之间分子相互作用所产生的粘附力尚未得到充分研究。因此，我们采用原子力显微镜直接测量VWF与GPIbα之间的粘附力。
我们使用原子力显微镜（AFM）在分子水平上测量VWF与GPIbα之间的粘附力。AFM悬臂梁涂有重组GPIbα的N端VWF结合位点，而盖玻片涂有天然VWF。
使用AFM测量分子水平的粘附力。在存在1μg/mL VWF的情况下，粘附力接近200 pN。根据高斯拟合分析，单键的粘附力可能为54或107 pN。
我们通过高斯拟合分析认为单键的粘附力可能为54 pN，这与光镊测得的结果（50 pN）非常接近。
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