维生素D受体基因Fok1多态性对补充钙和维生素D后骨量积累的影响。
Influence of Vitamin D Receptor Gene Fok1 Polymorphism on Bone Mass Accrual Post Calcium and Vitamin D Supplementation.
作者信息
Sanwalka Neha, Khadilkar Anuradha, Chiplonkar Shashi, Khatod Kavita, Phadke Nikhil, Khadilkar Vaman
机构信息
Department of Nutrition and Biostatistics, NutriCanvas, Mumbai, India.
Department of Growth and Endocrine, Hirabai Cowasji Jehangir Medical Research Institute, Jehangir Hospital, Old Building Basement, 32, Sassoon Road, Pune, Maharashtra, 411001, India.
出版信息
Indian J Pediatr. 2015 Nov;82(11):985-90. doi: 10.1007/s12098-015-1783-6. Epub 2015 May 15.
OBJECTIVE
To examine the association of vitamin D receptor (VDR) gene polymorphisms of the Fok1 locus on bone mass accrual in Indian girls used to a low calcium intake.
METHODS
An intervention trial was undertaken in 102 girls aged 8-16 y, attending a state run school in Pune city, India. All girls received 500 mg calcium daily and 30,000 IU of vitamin D3 quarterly for one year. Dietary calcium intake was evaluated. Bone mineral content (BMC), bone area (BA) and bone mineral density (BMD) were measured at total body using Dual Energy X-ray Absorptiometry (Lunar DPX-PRO). Polymorphisms of the Fok1 locus of the vitamin D Receptor (VDR) gene were detected using SYBR Green quantitative polymerase chain reaction.
RESULTS
The prevalence of Fok1 polymorphism was 43.1% (Ff), 9.8% (ff) and 47.1% (FF). At baseline, FF genotype had significantly lower BMD as compared to ff and Ff genotype (p < 0.05). At baseline, majority of girls (82.4%) were hypocalcemic with low calcium intake. Post-supplementation, FF genotype had significantly lower bone mass as compared to ff and Ff genotype. Significant increase in BMC [Ff (17.9%); ff (18.1%); FF (17.4%)], and BMD [Ff (5.4 %); ff (6.3%); FF (4.8%)] was observed post supplementation (p value < 0.05), though percentage increase in BMC and BMD was similar for three Fok1 polymorphisms (p > 0.1).
CONCLUSIONS
VDR gene polymorphism, as defined by Fok1 genotype had no positive influence on bone mass accrual in response to calcium supplementation.
目的
研究印度低钙摄入女孩中维生素D受体（VDR）基因Fok1位点多态性与骨量积累的相关性。
方法
对印度浦那市一所公立学校的102名8 - 16岁女孩进行了一项干预试验。所有女孩每天摄入500毫克钙，每季度摄入30,000国际单位维生素D3，持续一年。评估膳食钙摄入量。使用双能X线吸收法（Lunar DPX - PRO）测量全身的骨矿物质含量（BMC）、骨面积（BA）和骨矿物质密度（BMD）。采用SYBR Green定量聚合酶链反应检测维生素D受体（VDR）基因Fok1位点的多态性。
结果
Fok1多态性的发生率为43.1%（Ff）、9.8%（ff）和47.1%（FF）。基线时，与ff和Ff基因型相比，FF基因型的BMD显著更低（p < 0.05）。基线时，大多数女孩（82.4%）因钙摄入量低而血钙过低。补充后，与ff和Ff基因型相比，FF基因型的骨量显著更低。补充后观察到BMC [Ff（17.9%）；ff（18.1%）；FF（17.4%）]和BMD [Ff（5.4%）；ff（6.3%）；FF（4.8%）]有显著增加（p值 < 0.05），尽管三种Fok1多态性的BMC和BMD增加百分比相似（p > 0.1）。
结论
由Fok1基因型定义的VDR基因多态性对补钙后的骨量积累没有积极影响。
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