Bavi Hoda, Hosseini Seyed Ahmad, Ekrami Alireza, Alavi Seyed Mohammad, Malehi Amal Saki
Infectious and Tropical Diseases Research Center, Health Research Institute, Department of Nutrition, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Infectious and Tropical Diseases Research Center, Health Research Institute, Nutrition and Metabolic Diseases Research Center, Clinical Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Adv Biomed Res. 2024 Oct 28;13:102. doi: 10.4103/abr.abr_76_24. eCollection 2024.
Vitamin D leads to the activation of macrophages and limitation of intracellular growth of Mycobacterium tuberculosis. Vitamin D receptor (VDR) gene polymorphisms can facilitate the development of tuberculosis (TB). Therefore, the present study aimed to investigate the effect of vitamin D supplementation on response to treatment in patients with pulmonary TB for different VDR polymorphisms.
This semiexperimental study was carried out over a 2-year period on 73 patients (37 females; 36 males) who suffered from pulmonary TB. Vitamin D supplement at a daily dose of 800 IU was administered to the patients for 8 weeks. The serum levels of vitamin D were measured by electrochemiluminescence immunoassay. The polymerase chain reaction with a sequence-specific primers technique was used to determine the polymorphisms FOK1, Bsm 1, Apa 1, and Taq1 of VDR gene.
The mean serum levels of vitamin D increased significantly after the intervention (44.88 ng/ml vs 27.70 ng/ml, < 0.001). Those with FF genotype of Fok1 polymorphism had a higher chance of a positive response to treatment compared to the other genotypes ( = 0.044, 95% CI). Bsm1, Apa1, and Taq1 polymorphisms increased the treatment response, which was not statistically significant.
The results of this study showed that individuals harboring FF genotype of Fok1 polymorphism had a higher chance of a positive response to treatment with vitamin D compared with other genotypes. Therefore, vitamin D supplement can be an appropriate treatment considering the genetic characteristics of TB patients.
维生素D可导致巨噬细胞活化并限制结核分枝杆菌的细胞内生长。维生素D受体（VDR）基因多态性可促进结核病（TB）的发展。因此，本研究旨在探讨补充维生素D对不同VDR基因多态性的肺结核患者治疗反应的影响。
本半实验性研究对73例肺结核患者（37例女性；36例男性）进行了为期2年的研究。给予患者每日800 IU的维生素D补充剂，持续8周。采用电化学发光免疫分析法测定血清维生素D水平。采用序列特异性引物聚合酶链反应技术检测VDR基因的FOK1、Bsm 1、Apa 1和Taq1多态性。
干预后维生素D的平均血清水平显著升高（44.88 ng/ml对27.70 ng/ml，<0.001）。与其他基因型相比，Fok1多态性FF基因型的患者对治疗产生阳性反应的可能性更高（=0.044，95%CI）。Bsm1、Apa1和Taq1多态性增加了治疗反应，但无统计学意义。
本研究结果表明，与其他基因型相比，携带Fok1多态性FF基因型的个体对维生素D治疗产生阳性反应的可能性更高。因此，考虑到结核病患者的遗传特征，补充维生素D可能是一种合适的治疗方法。
