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SHRSP5/Dmcr大鼠反复冷应激诱导的机械性痛觉过敏的外周基因表达谱

Peripheral gene expression profile of mechanical hyperalgesia induced by repeated cold stress in SHRSP5/Dmcr rats.

作者信息

Kozaki Yasuko, Umetsu Rena, Mizukami Yukako, Yamamura Aya, Kitamori Kazuya, Tsuchikura Satoru, Ikeda Katsumi, Yamori Yukio

机构信息

College of Pharmacy, Kinjo Gakuin University, 2-1723 Ohmori, Moriyama-ku, Nagoya, 463-8521, Japan,

出版信息

J Physiol Sci. 2015 Sep;65(5):417-25. doi: 10.1007/s12576-015-0380-9. Epub 2015 May 14.

DOI:10.1007/s12576-015-0380-9
PMID:25972297
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10717666/
Abstract

Repeated cold stress (RCS) is known to transiently induce functional disorders associated with hypotension and hyperalgesia. In this study, we investigated the effects of RCS (24 and 4 °C alternately at 30-min intervals during the day and 4 °C at night for 2 days, followed by 4 °C on the next 2 consecutive nights) on the thresholds for cutaneous mechanical pain responses and on peripheral expression of "pain-related genes" in SHRSP5/Dmcr rats, which are derived from stroke-prone spontaneously hypertensive rats. To define genes peripherally regulated by RCS, we detected changes in the expression of pain-related genes in dorsal root ganglion cells by PCR-based cDNA subtraction analysis or DNA microarray analysis, and confirmed the changes by RT-PCR. We found significantly changed expression in eight pain-related genes (upregulated: Fyn, St8sia1, and Tac 1; downregulated: Ctsb, Fstl1, Itpr1, Npy, S100a10). At least some of these genes may play key roles in hyperalgesia induced by RCS.

摘要

已知反复冷应激（RCS）会短暂诱发与低血压和痛觉过敏相关的功能障碍。在本研究中，我们调查了RCS（白天每隔30分钟在24℃和4℃之间交替，夜间为4℃，持续2天，随后连续2个晚上保持在4℃）对SHRSP5/Dmcr大鼠皮肤机械性疼痛反应阈值以及“疼痛相关基因”外周表达的影响，SHRSP5/Dmcr大鼠源自易患中风的自发性高血压大鼠。为了确定受RCS外周调控的基因，我们通过基于PCR的cDNA消减分析或DNA微阵列分析检测背根神经节细胞中疼痛相关基因的表达变化，并通过RT-PCR进行了验证。我们发现八个疼痛相关基因的表达有显著变化（上调：Fyn、St8sia1和Tac 1；下调：Ctsb、Fstl1、Itpr1、Npy、S100a1

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