Shang Dan, Xiong Jun, Xiang Hong-Bing, Hao Yan, Liu Jiu-Hong
Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology University No. 1277 Jiefang Ave, Wuhan 430022, People's Republic of China.
Hepatobiliary Surgery Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology University No. 1277 Jiefang Ave, Wuhan 430022, People's Republic of China.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1996-2000. eCollection 2015.
This study was designed to assess whether MC4R signaling existed in vestibular nuclei modulated the activity of kidney by a virally mediated transsynaptic tracing study. Pseudorabies virus (PRV)-614 was injected into the kidney in adult male MC4R-green fluorescent protein (GFP) transgenic mice (n = 5). After a survival time of 5 days, the mice were assigned to humanely sacrifice, and the brainstem were removed and sectioned, and processed for PRV-614 visualization. The neurochemical phenotype of MC4R-GFP-positive neurons was identified using fluorescence immunocytochemical labeling. PRV-614/MC4R-GFP dual labeled neurons were detected in medial vestibular nuclei. Our findings support the hypothesis that there exist melanocortinergic circuits from medial vestibular nuclei to the kidney.
本研究旨在通过病毒介导的跨突触示踪研究，评估内侧前庭核中是否存在的MC4R信号传导调节肾脏活动。将伪狂犬病病毒（PRV）-614注射到成年雄性MC4R-绿色荧光蛋白（GFP）转基因小鼠（n = 5）的肾脏中。存活5天后，将小鼠进行安乐死，取出脑干并切片，然后进行PRV-614可视化处理。使用荧光免疫细胞化学标记鉴定MC4R-GFP阳性神经元的神经化学表型。在内侧前庭核中检测到PRV-614/MC4R-GFP双标记神经元。我们的研究结果支持以下假设：存在从内侧前庭核到肾脏的黑皮质素能回路。
