微小RNA-27a促进肾细胞癌的细胞增殖和转移。
miR-27a promotes cell proliferation and metastasis in renal cell carcinoma.
作者信息
Peng Hongjun, Wang Xianjun, Zhang Pei, Sun Tao, Ren Xianguo, Xia Zhengkun
机构信息
Department of Pediatrics, Jinling Hospital, Nanjing University School of Medicine 305 Zhongshan Road East, Nanjing 210002, P.R. China.
Department of Neurology, Linyi People's Hospital No. 49 Yizhou Road, Linyi 276000, Shandong, P.R. China.
出版信息
Int J Clin Exp Pathol. 2015 Feb 1;8(2):2259-66. eCollection 2015.
Abstract
miR-27a has been reported to exhibit abnormal expression in renal cell carcinoma (RCC), but the role of miR-27a in RCC remains unknown. In our study, up-regulation of miR-27a was validated by Real-time PCR analysis in 133 RCC samples. Overexpression of miR-27a promoted cell migration, invasion and proliferation in vitro, while its low expression exerted opposite effects. Kaplan-Meier analysis demonstrated that the patients with high expression of miR-27a had a worse overall and relapse-free survivals compared with those with low expression of miR-27a. Cox proportional hazards analyses showed that miR-27a expression was an independent prognostic factor for RCC patients. Collectively, our findings illustrate the promoting-cancer effect of miR-27a in RCC, suggesting that miR-27a could be a potential therapeutic target for RCC. Additionally, Kaplan-Meier analyses and Cox proportional regression analysis suggest that miR-27a may be a potential biomarker for predicting the survival of RCC patients.
摘要
据报道，miR-27a在肾细胞癌（RCC）中表达异常，但其在RCC中的作用尚不清楚。在我们的研究中，通过实时PCR分析在133例RCC样本中验证了miR-27a的上调。miR-27a的过表达促进了体外细胞迁移、侵袭和增殖，而其低表达则产生相反的效果。Kaplan-Meier分析表明，与miR-27a低表达的患者相比，miR-27a高表达的患者总生存期和无复发生存期更差。Cox比例风险分析表明，miR-27a表达是RCC患者的独立预后因素。总体而言，我们的研究结果说明了miR-27a在RCC中的促癌作用，表明miR-27a可能是RCC的潜在治疗靶点。此外，Kaplan-Meier分析和Cox比例回归分析表明，miR-27a可能是预测RCC患者生存的潜在生物标志物。
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