Shi Qiang, VandeBerg John L
Southwest National Primate Research Center, Texas Biomedical Research Institute San Antonio, Texas, 78227-5301.
Southwest National Primate Research Center, Texas Biomedical Research Institute San Antonio, Texas, 78227-5301 ; South Texas Diabetes and Obesity Institute, University of Texas Health Science Center, San Antonio - Regional Academic Health Center 80 Fort Brown Street, Brownsville, Texas 78520.
Am J Stem Cells. 2015 Mar 15;4(1):32-7. eCollection 2015.
Traditionally, CD34 positive cells are predominantly found in the umbilical cord and bone marrow, thus are considered as hematopoietic progenitors. Increasing evidence has suggested that the CD34+ cells represent a distinct subset of cells with enhanced progenitor activity; CD34 is a general marker of progenitor cells in a variety of cell types. Because the CD34 protein shows expression early on in hematopoietic and vascular-associated tissues, CD34+ cells have enormous potential as cellular agents for research and for clinical cell transplantation. Directed differentiation of embryonic stem cells will give rise to an inexhaustible supply of CD34+ cells, creating an exciting approach for biomedical research and for regenerative medicine. Here, we review the main methods that have been published for the derivation of CD34+ cells from embryonic stem cells; specifically those approaches the human and nonhuman primate stem cells. We summarize current status of this field, compare the methods used, and evaluate the issues in translating the bench science to bedside therapy.
传统上，CD34阳性细胞主要存在于脐带和骨髓中，因此被视为造血祖细胞。越来越多的证据表明，CD34+细胞代表了具有增强祖细胞活性的独特细胞亚群；CD34是多种细胞类型中祖细胞的通用标志物。由于CD34蛋白在造血和血管相关组织中早期就有表达，CD34+细胞作为研究和临床细胞移植的细胞制剂具有巨大潜力。胚胎干细胞的定向分化将产生源源不断的CD34+细胞，为生物医学研究和再生医学创造了一种令人兴奋的方法。在这里，我们回顾了已发表的从胚胎干细胞中获得CD34+细胞的主要方法；特别是那些针对人类和非人类灵长类干细胞的方法。我们总结了该领域的现状，比较了所使用的方法，并评估了将基础科学转化为床边治疗所存在的问题。
