一种来自内生真菌哈茨木霉的新型细胞毒性细胞松弛素。

A new cytotoxic cytochalasin from the endophytic fungus Trichoderma harzianum.

作者信息

Chen Huiqin, Daletos Georgios, Okoye Festus, Lai Daowan, Dai Haofu, Proksch Peter

出版信息

Nat Prod Commun. 2015 Apr;10(4):585-7.

Abstract

The new natural product 4]-hydroxy-deacetyl-18-deoxycytochalasin H (1), together with the known deacetyl-18-deoxycytochalasin H (2) and 18-deoxycytochalasin H (3) were obtained from the endophytic fungus Trichoderma harzianum isolated from leaves of Cola nitida. The structure of the new compound was unambiguously determined by 1D and 2D NMR spectroscopy, and by HRESIMS measurements, as well as by comparison with the literature. Compounds 1-3 showed potent cytotoxic activity against the murine lymphoma (L5178Y) cell line and against human ovarian cancer (A2780 sens and A2780 CisR) cell lines (IC50 0.19-6.97 µM). The A2780 cell lines included cisplatin-sensitive (sens) and -resistant (R) cells.

摘要

新的天然产物4]-羟基-去乙酰基-18-脱氧细胞松弛素H（1），与已知的去乙酰基-18-脱氧细胞松弛素H（2）和18-脱氧细胞松弛素H（3）一起，是从分离自可乐果叶片的内生真菌哈茨木霉中获得的。通过一维和二维核磁共振光谱、高分辨电喷雾电离质谱测量以及与文献对比，明确确定了新化合物的结构。化合物1-3对小鼠淋巴瘤（L5178Y）细胞系以及人卵巢癌（A2780敏感株和A2780顺铂耐药株）细胞系显示出强大的细胞毒性活性（半数抑制浓度为0.19-6.97 μM）。A2780细胞系包括顺铂敏感（sens）和耐药（R）细胞。