Ji Wengang, Ji Yuzhuo, Jin Qingqing, Tong Qingxiao, Tang Xinjing
State Key Laboratory of Natural and Biomimetic Drugs, The School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Analyst. 2015 Jul 7;140(13):4379-83. doi: 10.1039/c5an00549c.
A series of turn-on fluorescent probes with halogen acetyl amide at the 3-position of coumarin derivatives were synthesized. Fluorescence of these probes was efficiently quenched by heavy halogen atoms (Br and I, not Cl), which could be successfully used for selective detection of biothiols with the sensitivity of Cys > GSH > Hcy and much higher than thiol containing proteins. These represent the smallest fluorescence quenchers in designing fluorescent probes for detecting both endogenous and exogenous biothiols in living cells.
合成了一系列在香豆素衍生物3位带有卤代乙酰酰胺的开启型荧光探针。这些探针的荧光被重卤素原子（Br和I，而非Cl）有效淬灭，可成功用于生物硫醇的选择性检测，其对半胱氨酸（Cys）、谷胱甘肽（GSH）、同型半胱氨酸（Hcy）的检测灵敏度为Cys > GSH > Hcy，且远高于含硫醇的蛋白质。这些是用于检测活细胞内源性和外源性生物硫醇的荧光探针设计中最小的荧光淬灭剂。
