Hotchkiss Arland T, Nuñez Alberto, Strahan Gary D, Chau Hoa K, White André K, Marais Jannie P J, Hom Kellie, Vakkalanka Malathi S, Di Rong, Yam Kit L, Khoo Christina
†Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, United States.
‡Ocean Spray Cranberries, Inc., One Ocean Spray Drive, Lakeville-Middleboro, Massachusetts 02349, United States.
J Agric Food Chem. 2015 Jun 17;63(23):5622-33. doi: 10.1021/acs.jafc.5b00730. Epub 2015 Jun 9.
Cranberry juice has been recognized as a treatment for urinary tract infections on the basis of scientific reports of proanthocyanidin anti-adhesion activity against Escherichia coli as well as from folklore. Xyloglucan oligosaccharides were detected in cranberry juice and the residue remaining following commercial juice extraction that included pectinase maceration of the pulp. A novel xyloglucan was detected through tandem mass spectrometry analysis of an ion at m/z 1055 that was determined to be a branched, three hexose, four pentose oligosaccharide consistent with an arabino-xyloglucan structure. Two-dimensional nuclear magnetic resonance spectroscopy analysis provided through-bond correlations for the α-L-Araf (1→2) α-D-Xylp (1→6) β-D-Glcp sequence, proving the S-type cranberry xyloglucan structure. Cranberry xyloglucan-rich fractions inhibited the adhesion of E. coli CFT073 and UTI89 strains to T24 human bladder epithelial cells and that of E. coli O157:H7 to HT29 human colonic epithelial cells. SSGG xyloglucan oligosaccharides represent a new cranberry bioactive component with E. coli anti-adhesion activity and high affinity for type 1 fimbriae.
基于原花青素对大肠杆菌的抗黏附活性的科学报告以及民间传说，蔓越莓汁已被公认为是治疗尿路感染的一种方法。在蔓越莓汁以及商业榨汁后剩余的残渣（包括用果胶酶浸解果肉）中检测到了木葡聚糖寡糖。通过对质荷比为1055的离子进行串联质谱分析，检测到一种新型木葡聚糖，该离子被确定为一种与阿拉伯木葡聚糖结构一致的分支状三己糖、四戊糖寡糖。二维核磁共振光谱分析提供了α-L-阿拉伯呋喃糖（1→2）α-D-木糖（1→6）β-D-葡萄糖苷序列的键间相关性，证实了蔓越莓S型木葡聚糖结构。富含蔓越莓木葡聚糖的组分抑制了大肠杆菌CFT073和UTI89菌株对T24人膀胱上皮细胞的黏附以及大肠杆菌O157:H7对HT29人结肠上皮细胞的黏附。SSGG木葡聚糖寡糖代表了一种具有大肠杆菌抗黏附活性且对1型菌毛具有高亲和力的新型蔓越莓生物活性成分。
