Portevin Damien, Moukambi Félicien, Mpina Maxmillian, Bauer Asli, Haraka Frederick, Chachage Mkunde, Metzger Philipp, Saathoff Elmar, Clowes Petra, Ntinginya Nyanda E, Rachow Andrea, Hoelscher Michael, Reither Klaus, Daubenberger Claudia A, Geldmacher Christof
Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
NIMR-Mbeya Medical Research Centre, Mbeya, Tanzania; Department of Infectious Diseases and Tropical Medicine, Medical Centre of the University of Munich, Munich, Germany.
PLoS One. 2015 May 14;10(5):e0126716. doi: 10.1371/journal.pone.0126716. eCollection 2015.
It is well accepted that aging and HIV infection are associated with quantitative and functional changes of CMV-specific T cell responses. We studied here the expression of Mip-1β and the T cell maturation marker CD27 within CMVpp65-specific CD4(+) and CD8(+) T cells in relation to age, HIV and active Tuberculosis (TB) co-infection in a cohort of Tanzanian volunteers (≤ 16 years of age, n = 108 and ≥ 18 years, n = 79). Independent of HIV co-infection, IFNγ(+) CMVpp65-specific CD4(+) T cell frequencies increased with age. In adults, HIV co-infection further increased the frequencies of these cells. A high capacity for Mip-1β production together with a CD27(low) phenotype was characteristic for these cells in children and adults. Interestingly, in addition to HIV co-infection active TB disease was linked to further down regulation of CD27 and increased capacity of Mip-1β production in CMVpp65-specific CD4+ T cells. These phenotypic and functional changes of CMVpp65-specific CD4 T cells observed during HIV infection and active TB could be associated with increased CMV reactivation rates.
衰老和HIV感染与巨细胞病毒（CMV）特异性T细胞反应的数量和功能变化相关，这一点已得到广泛认可。我们在此研究了坦桑尼亚一组志愿者（≤16岁，n = 108；≥18岁，n = 79）中，CMV pp65特异性CD4(+)和CD8(+) T细胞内Mip-1β的表达以及T细胞成熟标志物CD27，这些均与年龄、HIV及活动性肺结核（TB）合并感染有关。独立于HIV合并感染情况，IFNγ(+) CMV pp65特异性CD4(+) T细胞频率随年龄增加。在成年人中，HIV合并感染进一步增加了这些细胞的频率。儿童和成年人中，这些细胞的特征是具有高Mip-1β产生能力以及CD27(low)表型。有趣的是，除了HIV合并感染外，活动性结核病还与CMV pp65特异性CD4+ T细胞中CD27的进一步下调以及Mip-1β产生能力增加有关。在HIV感染和活动性结核病期间观察到的CMV pp65特异性CD4 T细胞的这些表型和功能变化，可能与CMV再激活率增加有关。
