Illumina下一代测序揭示了变异拟鲹（鲈形目：鲳鲹科）的完整线粒体基因组并进行了系统发育分析。
Illumina next-generation sequencing reveals the complete mitochondrial genome of Psenopsis anomala (Perciformes: Centrolophidae) with phylogenetic consideration.
作者信息
Chen Huapu, Che Zhiwei, Li Jiantao, Dai Mingli, Xiang Ling, Deng Siping, Zhu Chunhua, Huang Hai, Li Guangli
机构信息
a Fisheries College, Guangdong Ocean University , Zhanjiang , China .
b Haikou Marine Environment Monitoring Center Station , Haikou , China , and.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3428-9. doi: 10.3109/19401736.2015.1022733. Epub 2015 May 14.
Using Illumina next-generation sequencing (NGS), the complete mitochondrial genome of the Psenopsis anomala was sequenced in the present study. The mitochondrial genome of P. anomala is 16,528 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The structure about gene order and composition of P. anomala mitochondrial genome is similar to those of most other vertebrates. The nucleotide compositions of the light strand in descending order is 29.18% of T, 27.97% of G, 27.06% of A, and 15.79% of C. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, other mitochondrial genes are encoded on the heavy strand. The phylogenetic analysis by maximum-likelihood (ML) method shown that the Psenopsis anomala was closer to Peprilus triacanthus in the phylogenetic relationship.
在本研究中，利用Illumina下一代测序（NGS）技术对黄斑鲾的完整线粒体基因组进行了测序。黄斑鲾的线粒体基因组长度为16,528 bp，由13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区组成。黄斑鲾线粒体基因组的基因顺序和组成结构与大多数其他脊椎动物相似。轻链的核苷酸组成按降序排列为：T占29.18%、G占27.97%、A占27.06%、C占15.79%。除了NADH脱氢酶亚基6（ND6）和8个tRNA基因外，其他线粒体基因都在重链上编码。通过最大似然法（ML）进行的系统发育分析表明，在系统发育关系上，黄斑鲾与三棘鲀更为接近。
Zotero 插件