Guan Bei, Guo Huifang, Zhou Zhijun
College of Life Sciences, Hebei University, Baoding, China.
Mitochondrial DNA B Resour. 2016 Jun 20;1(1):361-362. doi: 10.1080/23802359.2016.1168717.
Using Illumina next-generation sequencing (NGS), the complete mitochondrial genome (mitogenome) of was sequenced in the present study. The mitogenome of (Genbank accession no. KU885974) is 16,276 bp in size, had the typical invertebrate mitochondrial gene arrangement and containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA) and a control region. Except for the control region (868 bp), one novel larger intergenic (616 bp) was found between and . Phylogenetic results unambiguously support the monophyly of Phaneropterinae, although the gene order of two species different with other Tettigoniidae species. Using Illumina NGS platforms for mitogenome sequencing will provide rather essential and important DNA molecular data for the further phylogenetic analysis across major ensiferan lineages.
在本研究中,使用Illumina下一代测序(NGS)技术对[物种名称]的完整线粒体基因组(线粒体基因组)进行了测序。[物种名称]的线粒体基因组(Genbank登录号KU885974)大小为16,276 bp,具有典型的无脊椎动物线粒体基因排列,包含13个蛋白质编码基因(PCG)、22个转运RNA基因(tRNA)、2个核糖体RNA基因(rRNA)和一个控制区。除控制区(868 bp)外,在[基因名称1]和[基因名称2]之间发现了一个新的较大的基因间隔区(616 bp)。系统发育结果明确支持扇螽亚科的单系性,尽管两个[物种名称]物种的基因顺序与其他螽斯科物种不同。使用Illumina NGS平台进行线粒体基因组测序将为跨主要直翅目谱系的进一步系统发育分析提供相当重要的DNA分子数据。