Liu Y L, Cheng M, Jiang M F, Wang Yo, Wang Jie, Fu X H
Genetika. 2014 Dec;50(12):1440-7. doi: 10.7868/s0016675814120066.
Eight goat breeds from Chengdu, Lezhi, Jintang, Baiyu, Yingshan, Hejiang, Jiangan, and Jialing in Sichuan, China were analyzed for genetic diversity using AFLP markers. A total of 261 individuals were analyzed using eight selective AFLP primer combinations which generated 174 discernible fragments, of which 80 were polymorphic with an average of 27.15 bands per primer pair. Shannon's gene diversity index among breeds ranged from 0.0888 to 0.2289, and the highest and the lowest gene diversity index among breeds were the Yingshan Black goat (0.2289) and the Baiyu Black goat (0.0888) respectively. The genetic distance (DR) among eight goat breeds were calculated by Rogers' genetic distance. Based on the analytical results, systematic trees were constructed by using the unweighted pair-group method with arithmetic average (UPGMA) and the neighbor joining (NJ) methods. Eight breeds were divided into two main clusters by using cluster analysis, one of which including two subclusters. The clustering results agreed well with the origin of the goats and geographic distribution. The results implied that AFLP markers were informative and could provide significant insights for genetic diversity research and genetic resource conservation of indigenous goats.
利用AFLP标记对来自中国四川省成都、乐至、金堂、白玉、营山、合江、江安和嘉陵的8个山羊品种进行了遗传多样性分析。使用8对选择性AFLP引物组合对总共261个个体进行了分析，共产生了174个可分辨的片段，其中80个具有多态性，每对引物平均产生27.15条带。品种间的香农基因多样性指数在0.0888至0.2289之间，品种间基因多样性指数最高和最低的分别是营山黑山羊（0.2289）和白玉黑山羊（0.0888）。采用罗杰斯遗传距离计算8个山羊品种间的遗传距离（DR）。根据分析结果，运用非加权算术平均法（UPGMA）和邻接法（NJ）构建了系统树。通过聚类分析将8个品种分为两个主要类群，其中一个类群包含两个亚类群。聚类结果与山羊的起源和地理分布吻合良好。结果表明，AFLP标记具有信息性，可为本地山羊的遗传多样性研究和遗传资源保护提供重要见解。
