Matsuda T, Kondo A, Makino K, Akutsu T
Department of Bioengineering, National Cardiovascular Center, Osaka, Japan.
ASAIO Trans. 1989 Jul-Sep;35(3):677-9. doi: 10.1097/00002480-198907000-00165.
The authors present a novel artificial matrix with high cell adhesion and growth rate that was produced by chemical fixation of an RGD-containing peptide (Arg-Gly-Asp) on a polyvinyl alcohol (PVA) surface. The logical sequence behind the molecular design of an artificial matrix that mimics natural adhesive proteins, such as fibronectin, is based on the fact that an RGD tripeptidyl amino sequence is the minimal common adhesion site of adhesive proteins. Activation of surface hydroxyl groups by carbonyl diimidazole successfully incorporated GRGDSP (Gly-Arg-Gly-Asp-Ser-Pro) onto PVA films. The resultant film surface was found to be bioactive, molecularly recognizing the adhesive receptor of bovine endothelial cells. Thus, the artificial matrix mimics active adhesion sites and serves as a novel artificial matrix that may be useful for cell culture, tissue-compatible implants, and hybrid artificial organs.
作者展示了一种新型人工基质，其具有高细胞黏附性和生长速率，该基质是通过将含RGD的肽（精氨酸-甘氨酸-天冬氨酸）化学固定在聚乙烯醇（PVA）表面而制备的。模仿天然黏附蛋白（如纤连蛋白）的人工基质分子设计背后的逻辑顺序基于这样一个事实，即RGD三肽基氨基酸序列是黏附蛋白的最小共同黏附位点。通过羰基二咪唑活化表面羟基成功地将GRGDSP（甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸-脯氨酸）整合到PVA薄膜上。结果发现所得薄膜表面具有生物活性，能够分子识别牛内皮细胞的黏附受体。因此，这种人工基质模仿了活性黏附位点，可作为一种新型人工基质，可能对细胞培养、组织相容性植入物和混合人工器官有用。
