Sugawara T, Matsuda T
Department of Bioengineering, National Cardiovascular Center, Research Institute, Osaka, Japan.
J Biomed Mater Res. 1995 Sep;29(9):1047-52. doi: 10.1002/jbm.820290904.
A photoreactive octapeptide (Gly-Gly-Gly-Arg-Gly-Asp-Ser-Pro), into which the cell adhesive tripeptidyl sequence Arg-Gly-Asp (RGD) was incorporated, was derivatized with 4-azidobenzoyloxysuccinimide at its N-terminal. Photochemical surface immobilization of the RGD peptide on poly (vinyl alcohol) (PVA) was performed by adsorption and subsequent ultraviolet (UV) irradiation and was evidenced by electron spectra for chemical analysis (ESCA) measurements. Bovine endothelial cells (ECs) adhered and spread well on the RGD peptide-derivatized surfaces in a biologically specific manner. The use of a photomask during UV irradiation enabled preparation of a two-dimensional tissue with micron-order precision. Thus surface immobilization of a photoreactive, cell-adhesive peptide easily can create a cell attachment surface at a desired portion or region of a polymer surface or device.
一种光反应性八肽（甘氨酸-甘氨酸-甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸-脯氨酸），其中掺入了细胞黏附性三肽序列精氨酸-甘氨酸-天冬氨酸（RGD），在其N端用4-叠氮苯甲酰氧基琥珀酰亚胺进行衍生化。通过吸附及随后的紫外线（UV）照射，将RGD肽光化学固定在聚乙烯醇（PVA）表面，并通过化学分析电子能谱（ESCA）测量得到证实。牛内皮细胞（ECs）以生物特异性方式在RGD肽衍生化表面良好地黏附与铺展。在UV照射期间使用光掩膜能够以微米级精度制备二维组织。因此，光反应性细胞黏附肽的表面固定能够轻易地在聚合物表面或装置的所需部分或区域创建细胞附着表面。
