Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Infectious Diseases Hospital, Ministry of Health, Kuwait.
J Med Virol. 2015 Sep;87(9):1521-6. doi: 10.1002/jmv.24212. Epub 2015 May 14.
Mutations associated with resistance to antiretroviral therapy are a major cause of failure to treatment, and surveillance for the emergence of HIV resistance became a component of all antiretroviral treatment programs. As transmission of resistant viruses to newly infected persons is possible, we aimed to determine the prevalence of primary mutations associated with antiretroviral resistance among treatment-naïve patients, with respect to HIV subtype. Viral RNA was extracted from plasma samples of 43 treatment-naïve patients. Protease (PR) and reverse transcriptase (RT) regions were amplified and sequenced using the TRUGENE HIV-1 Genotyping Assay. A phylogenetic analysis was performed for HIV subtype assignment. Complete sequence information could be obtained for 35 patients. A total of ten different HIV-1 subtypes and recombinant forms were found in Kuwait with predominance of subtypes B, C, and CRF01_AE. A62V and A98G were non-polymorphic resistance-associated mutations (RAMs) detected in the RT region of two and three patients, respectively. Non-polymorphic mutations associated with resistance to protease inhibitors were not detected. Our results support continuous surveillance of RAMs in newly infected individuals to assess the effectiveness of first-line antiretroviral regimen available in Kuwait.
与抗逆转录病毒治疗耐药相关的突变是治疗失败的主要原因，HIV 耐药性的监测已成为所有抗逆转录病毒治疗方案的组成部分。由于耐药病毒可能传播给新感染的人，我们旨在确定与 HIV 亚型相关的初治患者中与抗逆转录病毒耐药相关的主要突变的流行率。从 43 名初治患者的血浆样本中提取病毒 RNA。使用 TRUGENE HIV-1 基因分型检测试剂盒扩增和测序蛋白酶 (PR) 和逆转录酶 (RT) 区。进行了 HIV 亚型分配的系统发育分析。可以获得 35 名患者的完整序列信息。在科威特发现了总共十种不同的 HIV-1 亚型和重组形式，其中以亚型 B、C 和 CRF01_AE 为主。在 RT 区分别检测到两名和三名患者的非多态性耐药相关突变 (RAM) A62V 和 A98G。未检测到与蛋白酶抑制剂耐药相关的非多态性突变。我们的结果支持对新感染个体中 RAM 进行持续监测，以评估科威特现有一线抗逆转录病毒方案的有效性。
