Molecular Biology Laboratory, Blood Transfusion Center, Botucatu Medical School, Sao Paulo State University, UNESP, São Paulo, Brazil.
Department of Bioprocess and Biotechnology, School of Agricultural Sciences, Lageado Experiment Station. Sao Paulo State University, UNESP, Botucatu-SP, Brazil.
J Med Virol. 2015 Oct;87(10):1677-81. doi: 10.1002/jmv.24233. Epub 2015 May 14.
To evaluate the associations of HPA polymorphisms -1, -3, and -5 with HIV/HCV coinfection were included in this study 60 HIV/HCV-coinfected patients from the Sao Paulo State health service centers. Data reported by Verdichio-Moraes et al. (2009: J. Med Virol 81:757-759) were used as the non-infected and HCV monoinfected groups. Human Platelet Polymorphism genotyping was performed in 60 Patients co-infected with HIV/HCV by PCR-SSP or PCR-RFLP. HIV subtyping and HCV genotyping was performed by RT-PCR followed sequencing. The data analyses were performed using the χ2 test or Fisher's Exact Test and the logistic regression model. Patients coinfected with HIV/HCV presented HCV either genotype 1 (78.3%) or non-1 (21.7%) and HIV either subtype B (85.0%) or non-B (15%). The Human Platelet Polymorphism-1a/1b genotype was more frequent (P < 0.05) in HIV/HCV coinfection than in HCV monoinfection and the allelic frequency of Human Platelet Polymorphism-5b in the Patients coinfected with HIV/HCV was higher (P < 0.05) than in HCV monoinfected cases and non-infected individuals. These data suggest that the presence of specific HPA allele on platelets could favor the existence of coinfection. On the other hand, Human Platelet Polymorphism-5a/5b was more frequent (P < 0.05) in HIV/HCV coinfected and HCV monoinfected groups than in the non-infected individuals, suggesting that this platelet genotype is related to HCV infection, regardless of HIV presence. Results suggest that the Human Platelet Polymorphism profile in HIV/HCV coinfected individuals differs from the one of both HCV monoinfected and non-infected population. So, the Human Platelet Polymorphism can be a genetic marker associated with HIV/HCV coinfection.
为了评估 HPA 多态性-1、-3 和-5 与 HIV/HCV 合并感染的相关性，本研究纳入了 60 名来自圣保罗州卫生服务中心的 HIV/HCV 合并感染患者。Verdichio-Moraes 等人（2009 年：J. Med Virol 81:757-759）报告的数据被用作未感染和 HCV 单感染组。通过 PCR-SSP 或 PCR-RFLP 对 60 名 HIV/HCV 合并感染患者进行人类血小板多态性基因分型。通过 RT-PCR 后测序进行 HIV 亚型和 HCV 基因分型。使用 χ2 检验或 Fisher's Exact Test 和逻辑回归模型进行数据分析。感染 HIV/HCV 的患者同时感染 HCV 基因型 1（78.3%）或非 1 型（21.7%）和 HIV 亚型 B（85.0%）或非 B（15.0%）。与 HCV 单感染相比，HIV/HCV 合并感染患者中人类血小板多态性-1a/1b 基因型更为常见（P<0.05），而感染 HIV/HCV 的患者血小板中人类血小板多态性-5b 的等位基因频率也高于 HCV 单感染和未感染个体（P<0.05）。这些数据表明，血小板上特定 HPA 等位基因的存在可能有利于合并感染的发生。另一方面，人类血小板多态性-5a/5b 在 HIV/HCV 合并感染和 HCV 单感染组中比未感染个体更为常见（P<0.05），表明这种血小板基因型与 HCV 感染有关，而与 HIV 的存在无关。结果表明，HIV/HCV 合并感染患者的人类血小板多态性谱与 HCV 单感染和未感染人群的谱不同。因此，人类血小板多态性可以作为与 HIV/HCV 合并感染相关的遗传标志物。
